Kok Menno, Bron Guillaume, Erni Bernhard, Mukhija Seema
Département de Génétique et Microbiologie, CMU, 9, Avenue de Champel, CH-1211 Genève, Switzerland.
Departement für Chemie und Biochemie, Freiestrasse 3, Universität Bern, CH-3012, Bern, Switzerland.
Microbiology (Reading). 2003 Sep;149(Pt 9):2645-2652. doi: 10.1099/mic.0.26406-0.
The phosphoenolpyruvate : sugar phosphotransferase system (PTS) catalyses translocation with concomitant phosphorylation of sugars and hexitols and it regulates metabolism in response to the availability of carbohydrates. The PTS forms an interface between energy and signal transduction and its inhibition is likely to have pleiotropic effects. It is present in about one-third of bacteria with fully sequenced genomes, including many common pathogens, but does not occur in eukaryotes. Enzyme I (ptsI) is the first component of the divergent protein phosphorylation cascade. ptsI deletions were constructed in Salmonella typhimurium, Staphylococcus aureus and Haemophilus influenzae and virulence of the mutants was characterized in an intraperitoneal mouse model. The log(attenuation) values were 2.3, 1.4 and 0.9 for the Sal. typhimurium, Sta. aureus and H. influenzae ptsI mutants, respectively. The degree of attenuation is correlated with the complexity of the respective PTS, which comprises approximately 40 components in Sal. typhimurium, but only 5 in H. influenzae.
糖磷酸转移酶系统（PTS）催化糖类和己糖醇的易位并伴随磷酸化，它根据碳水化合物的可利用性调节新陈代谢。PTS形成了能量与信号转导之间的界面，其抑制作用可能具有多效性。它存在于约三分之一具有全基因组测序的细菌中，包括许多常见病原体，但在真核生物中不存在。酶I（ptsI）是不同蛋白磷酸化级联反应的第一个组分。在鼠伤寒沙门氏菌、金黄色葡萄球菌和流感嗜血杆菌中构建了ptsI缺失突变体，并在腹腔小鼠模型中对突变体的毒力进行了表征。鼠伤寒沙门氏菌、金黄色葡萄球菌和流感嗜血杆菌ptsI突变体的对数（减毒）值分别为2.3、1.4和0.9。减毒程度与各自PTS的复杂性相关，鼠伤寒沙门氏菌的PTS约由40个组分组成，而流感嗜血杆菌的PTS仅由5个组分组成。
