Fu Sai Chuen, Qin Lin, Leung Chi Kam, Chan Barbara Pui, Chan Kai Ming
Department of Orthopaedics and Traumatology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong.
Can J Appl Physiol. 2003 Jun;28(3):370-81. doi: 10.1139/h03-027.
The biphasic effects of exercise training on the immune system have been studied extensively and represented by the well-known J-shaped curve with respect to training intensity. However, the relationship and interactions between "beneficial" exercise training and "harmful" strenuous exercise have not been researched. This study was designed to determine whether regular moderate exercise training could affect the changes of percentage of T-lymphocytes induced by a single bout of strenuous exercise. A protocol to run uphill on a 10 degrees tilted treadmill for 4 weeks was employed as moderate exercise training in mice, while a sedentary control group of mice was exposed to the same handling stress without training. The trained and untrained mice were then exposed to a single bout of strenuous exercise until exhaustion. Total leukocytes were collected from spleen and peripheral blood at 0 hr, 3 hrs, and 24 hrs postexhaustion, as well as from the control groups. Flow cytometric analyses were conducted to determine the percentages of selected leukocyte populations. It was demonstrated that moderate exercise training prevented the decrease of CD4+ but stimulated the increase of CD25+ CD8+ T-lymphocytes induced by a single bout of strenuous exercise, indicating an adaptive response that can affect changes of leukocyte subpopulations.
运动训练对免疫系统的双相效应已得到广泛研究，并以著名的J形曲线来表示训练强度与免疫系统的关系。然而，“有益的”运动训练与“有害的”剧烈运动之间的关系和相互作用尚未得到研究。本研究旨在确定定期适度运动训练是否会影响单次剧烈运动诱导的T淋巴细胞百分比变化。采用在10度倾斜跑步机上爬坡跑4周的方案作为小鼠的适度运动训练，而久坐不动的对照组小鼠则在不训练的情况下接受相同的处理应激。然后，对经过训练和未经过训练的小鼠进行单次剧烈运动直至精疲力竭。在力竭后0小时、3小时和24小时以及从对照组收集脾脏和外周血中的总白细胞。进行流式细胞术分析以确定选定白细胞群体的百分比。结果表明，适度运动训练可防止单次剧烈运动诱导的CD4 +细胞减少，但刺激CD25 + CD8 + T淋巴细胞增加，表明这是一种可影响白细胞亚群变化的适应性反应。
