运用多重聚合酶链反应鉴定参与人类血源性感染的金黄色葡萄球菌黏附素。
Use of multiplex PCR to identify Staphylococcus aureus adhesins involved in human hematogenous infections.
作者信息
Tristan Anne, Ying Liu, Bes Michele, Etienne Jerome, Vandenesch Francois, Lina Gerard
机构信息
Centre National de Référence des Staphylocoques, INSERM E0230, IFR62 Laennec, 69372 Lyon Cédex 08, France.
出版信息
J Clin Microbiol. 2003 Sep;41(9):4465-7. doi: 10.1128/JCM.41.9.4465-4467.2003.
Abstract
We have developed a multiplex PCR procedure to determine the distribution of nine adhesin genes in Staphylococcus aureus isolates. Only genes encoding bone sialoprotein binding protein and fibronectin binding protein B were significantly associated with hematogenous osteomyelitis/arthritis and native-valve endocarditis, respectively, suggesting their involvement in hematogenous tissue infections.
摘要
我们开发了一种多重聚合酶链反应程序，以确定金黄色葡萄球菌分离株中9种粘附素基因的分布。仅编码骨唾液蛋白结合蛋白和纤连蛋白结合蛋白B的基因，分别与血源性骨髓炎/关节炎和天然瓣膜心内膜炎显著相关，这表明它们参与了血源性组织感染。
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