用副品红共振光散射法测定脱氧核糖核酸
[Determination of deoxyribonucleic acid with pararosaniline by a resonance light-scattering method].
作者信息
Xiang Hai-yan, Chen Xiao-ming, Zhou Di-wu
机构信息
Printed Circuit Board Factory, Zhuzhou Electric Locomotive Institute, Zhuzhou 412001, China.
出版信息
Guang Pu Xue Yu Guang Pu Fen Xi. 2002 Dec;22(6):1051-3.
PMID:12914197
Abstract
The method is based on the enhancement effect of the resonance light-scattering (RLS) of pararosaniline by DNA. In the pH rang of 0.5-1.5, the enhanced intensity of pararosaniline RLS at 355 nm is proportional to the concentration of DNA in the rang of 0.20-15 micrograms.mL-1. The detection limit is 36 ng.mL-1. This method is simple, rapid and has been applied satisfactorily to the determination of DNA in mixed samples.
摘要
该方法基于DNA对副品红共振光散射（RLS）的增强作用。在0.5 - 1.5的pH范围内，355nm处副品红RLS的增强强度与0.20 - 15微克·毫升⁻¹范围内的DNA浓度成正比。检测限为36纳克·毫升⁻¹。该方法简单、快速，已成功应用于混合样品中DNA的测定。
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