Tasaka K, Shorter R G, Code C F
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Methods Find Exp Clin Pharmacol. 1992 Dec;14(10):799-804.
Leukocytes were labelled by intravenous injection of tritiated thymidine (3H-thymidine) in dogs to discover the source of the increased number of neutrophils in the circulating blood after injection of histamine in beeswax. Dogs with normal hemograms were given 1.0 mCi/kg of 3H-thymidine alone, and in different sequences, with histamine in beeswax. When 3H-thymidine was given during maintained histamine leukocytosis, labelled granulocytes appeared in and disappeared from the blood earlier than in control tests and the number of labelled cells was greater in the histamine-treated animals. Administration of histamine in beeswax 3 days after injection of 3H-thymidine also induced the premature appearance and disappearance of labelled neutrophils in the circulating blood. It was concluded that leukocytosis induced by the chronic action of histamine is due to 1) stimulated proliferation and differentiation of neutrophil precursor cells in the bone marrow and 2) the release of mature leukocytes from the bone marrow.
通过给狗静脉注射氚标记的胸腺嘧啶核苷（³H - 胸腺嘧啶核苷）来标记白细胞，以探究在注射蜂蜡组胺后循环血液中嗜中性粒细胞数量增加的来源。给血细胞计数正常的狗单独注射1.0毫居里/千克的³H - 胸腺嘧啶核苷，并以不同顺序与蜂蜡组胺一起注射。当在组胺引起的白细胞增多持续期间给予³H - 胸腺嘧啶核苷时，标记的粒细胞在血液中出现和消失的时间比对照试验中更早，并且在接受组胺治疗的动物中标记细胞的数量更多。在注射³H - 胸腺嘧啶核苷3天后给予蜂蜡组胺也会导致循环血液中标记的嗜中性粒细胞过早出现和消失。得出的结论是，组胺的慢性作用所诱导的白细胞增多是由于：1）骨髓中嗜中性粒细胞前体细胞的增殖和分化受到刺激；2）成熟白细胞从骨髓中释放出来。
