Grimaldi Júnior G, Kreutzer R D, Hashiguchi Y, Gomez E A, Mimory T, Tesh R B
Departamento de Imunologia, Instituto Oswaldo Cruz, RJ, Brasil.
Mem Inst Oswaldo Cruz. 1992 Apr-Jun;87(2):221-8. doi: 10.1590/s0074-02761992000200009.
Characterization is given of a new parasite, Leishmania equatorensis sp. n., which was isolated from the viscera of a sloth (Choloepus hoffmanni) and a squirrel (Sciurus granatensis), captured in humid tropical forest on the Pacific Coast of Ecuador. Data based on biological and molecular criteria, as well as numerical zymotaxonomical analysis, indicate that this parasite is a new species of the L. braziliensis complex. L. equatorensis is clearly distinguishable from all other known species within this complex, using the following molecular criteria: reactivity patterns with specific monoclonal antibodies, isoenzyme electrophoresis, and restriction-endonuclease fragment patterns of kinetoplast DNA (k-DNA).
对一种新寄生虫——赤道利什曼原虫（Leishmania equatorensis sp. n.）进行了特征描述。该寄生虫是从厄瓜多尔太平洋沿岸潮湿热带森林中捕获的一只树懒（霍氏树懒Choloepus hoffmanni）和一只松鼠（松鼠Sciurus granatensis）的内脏中分离出来的。基于生物学和分子标准以及数值酶分类分析的数据表明，这种寄生虫是巴西利什曼原虫复合体的一个新物种。利用以下分子标准，赤道利什曼原虫可与该复合体中所有其他已知物种明显区分开来：与特定单克隆抗体的反应模式、同工酶电泳以及动基体DNA（k-DNA）的限制性内切酶片段模式。
