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单纯疱疹病毒DNA聚合酶基因一个独特保守区域内的点突变赋予了耐药性。

A point mutation within a distinct conserved region of the herpes simplex virus DNA polymerase gene confers drug resistance.

作者信息

Hwang C B, Ruffner K L, Coen D M

机构信息

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.

出版信息

J Virol. 1992 Mar;66(3):1774-6. doi: 10.1128/JVI.66.3.1774-1776.1992.

DOI:10.1128/JVI.66.3.1774-1776.1992
PMID:1310779
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC240935/
Abstract

We have shown that a drug-resistant mutant from a clinical isolate of herpes simplex virus contains a single point mutation in the DNA polymerase gene that confers resistance to both acyclovir and foscarnet. The mutated amino acid is located within a distinct conserved region shared among alpha-like DNA polymerases which we designate region VII. We infer that these conserved sequences are directly or indirectly involved in the recognition and binding of nucleotide and PPi substrates.

摘要

我们已经证明，从单纯疱疹病毒临床分离株中获得的耐药突变体在DNA聚合酶基因中含有一个单点突变，该突变赋予了对阿昔洛韦和膦甲酸的耐药性。突变的氨基酸位于α样DNA聚合酶共有的一个独特保守区域内，我们将其命名为区域VII。我们推断，这些保守序列直接或间接参与核苷酸和焦磷酸底物的识别和结合。

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