Gropp R, Gropp F, Betlach M C
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Proc Natl Acad Sci U S A. 1992 Feb 15;89(4):1204-8. doi: 10.1073/pnas.89.4.1204.
The sedimentation behavior of the halobacterial 7S RNA and bacterioopsin mRNA was assessed after application of total cell lysates to sucrose gradients. These two RNAs cosedimented predominantly with membrane-bound polysomes, and the quantity of 7S RNA bound to the ribosomes was directly correlated with the expression of bacterioopsin. Puromycin treatment released the 7S RNA from the polysomes, indicating that it is transiently associated with protein translation. We suggest that halobacteria contain a signal-recognition-like particle involved in translation of membrane-associated proteins.
将全细胞裂解物应用于蔗糖梯度后，评估了嗜盐菌7S RNA和细菌视紫红质mRNA的沉降行为。这两种RNA主要与膜结合多核糖体共沉降，与核糖体结合的7S RNA的量与细菌视紫红质的表达直接相关。嘌呤霉素处理使7S RNA从多核糖体上释放出来，表明它与蛋白质翻译短暂相关。我们认为嗜盐菌含有一种参与膜相关蛋白翻译的信号识别样颗粒。
