Moritz A, Goebel W
Nucleic Acids Res. 1985 Oct 11;13(19):6969-79. doi: 10.1093/nar/13.19.6969.
The 7S RNA is an abundant nonribosomal RNA in H. halobium and other halobacteria. A specific 7S RNA gene probe shows high homology to genomic DNA of all halobacteria tested but not to those of several other archaebacteria, eubacteria and eukaryotes. All halobacterial genomes seem to carry a single copy of the 7S RNA gene. The coding region of the 7S RNA gene is highly G+C rich whereas the 5'- and 3'-noncoding regions possess a rather low G+C content. An extended double stranded structure for the 7S RNA is deduced from its nucleotide sequence. The 7S RNA of H. halobium (304 nucleotides) resembles in size and structure the 7S-L RNA from mammalian cells and shares with it a sequence homology of about 50% when arranged in a colinear fashion. The similarities in sequence are found particularly at the 3'- and 5'-termini. No similarity was detected between the 7S RNA from H. halobium and the nonribosomal 6S RNA from Escherichia coli.
7S RNA是嗜盐栖热菌及其他嗜盐细菌中一种丰富的非核糖体RNA。一种特定的7S RNA基因探针显示，它与所有测试的嗜盐细菌的基因组DNA具有高度同源性，但与其他几种古细菌、真细菌和真核生物的基因组DNA没有同源性。所有嗜盐细菌的基因组似乎都携带一份7S RNA基因拷贝。7S RNA基因的编码区富含G+C，而5'和3'非编码区的G+C含量相当低。根据其核苷酸序列推断出7S RNA具有延伸的双链结构。嗜盐栖热菌的7S RNA（304个核苷酸）在大小和结构上与哺乳动物细胞的7S-L RNA相似，当以共线方式排列时，它们的序列同源性约为50%。序列相似性尤其在3'和5'末端被发现。未检测到嗜盐栖热菌的7S RNA与大肠杆菌的非核糖体6S RNA之间存在相似性。
