新生肽作为细菌中多核糖体与膜的唯一附着方式。
Nascent peptide as sole attachment of polysomes to membranes in bacteria.
作者信息
Smith W P, Tai P C, Davis B D
出版信息
Proc Natl Acad Sci U S A. 1978 Feb;75(2):814-7. doi: 10.1073/pnas.75.2.814.
Abstract
When membrane-polysome complexes from Escherichia coli were treated with puromycin, at various Mg2+ and K+ concentrations, the bulk of the ribosomes were released from the membrane. Moreover, many released ribosomes released ribosomes remained attached to mRNA (pseudopolysomes). These results suggest that ribosomes are attached to the membrane in bacteria solely by their nascent chain. Without a direct attachment the conformational changes associated with chain elongation on the ribosome cannot force the growing chain through the membrane, and so alternative sources of energy for the transfer must be considered.
摘要
当用嘌呤霉素在不同的镁离子和钾离子浓度下处理来自大肠杆菌的膜 - 多核糖体复合物时，大部分核糖体从膜上释放出来。此外，许多释放出来的核糖体仍附着于信使核糖核酸（假多核糖体）。这些结果表明，细菌中的核糖体仅通过其新生肽链附着于膜上。没有直接附着，核糖体上与肽链延伸相关的构象变化就无法迫使正在生长的肽链穿过膜，因此必须考虑转移过程中的其他能量来源。
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