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一种大肠杆菌DNA拓扑异构酶I突变体具有一个补偿性突变，该突变改变了DNA促旋酶A蛋白功能域之间的两个氨基酸残基。

An Escherichia coli DNA topoisomerase I mutant has a compensatory mutation that alters two residues between functional domains of the DNA gyrase A protein.

作者信息

Oram M, Fisher L M

机构信息

Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, University of London, United Kingdom.

出版信息

J Bacteriol. 1992 Jun;174(12):4175-8. doi: 10.1128/jb.174.12.4175-4178.1992.

DOI:10.1128/jb.174.12.4175-4178.1992
PMID:1317847
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC206131/
Abstract

Nucleotide sequence analysis revealed that the compensatory gyrA mutation in Escherichia coli DM750 affects DNA supercoiling by interchanging the identities of Ala-569 and Thr-586 in the DNA gyrase A subunit. These residues flank Arg-571, a site for trypsin cleavage that splits gyrase A protein between DNA breakage-reunion and DNA-binding domains. The putative interdomain locations of the DM750 mutation and that of E. coli DM800 (in gyrase B protein) suggests that these compensatory mutations may reduce DNA supercoiling activity by altering allosteric interactions in the gyrase complex.

摘要

核苷酸序列分析表明，大肠杆菌DM750中的补偿性gyrA突变通过互换DNA促旋酶A亚基中Ala-569和Thr-586的身份来影响DNA超螺旋。这些残基位于Arg-571两侧，Arg-571是胰蛋白酶切割位点，可在DNA断裂-重连和DNA结合结构域之间切割促旋酶A蛋白。DM750突变和大肠杆菌DM800（在促旋酶B蛋白中）的假定结构域间位置表明，这些补偿性突变可能通过改变促旋酶复合物中的变构相互作用来降低DNA超螺旋活性。

相似文献

1
An Escherichia coli DNA topoisomerase I mutant has a compensatory mutation that alters two residues between functional domains of the DNA gyrase A protein.一种大肠杆菌DNA拓扑异构酶I突变体具有一个补偿性突变，该突变改变了DNA促旋酶A蛋白功能域之间的两个氨基酸残基。
J Bacteriol. 1992 Jun;174(12):4175-8. doi: 10.1128/jb.174.12.4175-4178.1992.
2
Regulation of DNA supercoiling in Escherichia coli: genetic basis of a compensatory mutation in DNA gyrase.大肠杆菌中DNA超螺旋的调控：DNA回旋酶补偿性突变的遗传基础。
FEBS Lett. 1989 Aug 14;253(1-2):67-70. doi: 10.1016/0014-5793(89)80931-7.
3
gyrB-225, a mutation of DNA gyrase that compensates for topoisomerase I deficiency: investigation of its low activity and quinolone hypersensitivity.gyrB - 225，一种补偿拓扑异构酶I缺陷的DNA促旋酶突变：对其低活性和喹诺酮超敏反应的研究
J Mol Biol. 2001 Jun 22;309(5):1219-31. doi: 10.1006/jmbi.2001.4733.
4
Mechanism of DNA gyrase-mediated illegitimate recombination: characterization of Escherichia coli gyrA mutations that confer hyper-recombination phenotype.DNA旋转酶介导的异常重组机制：对赋予高重组表型的大肠杆菌gyrA突变的表征
J Mol Biol. 1999 Jun 11;289(3):447-58. doi: 10.1006/jmbi.1999.2758.
5
Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes.大肠杆菌DNA拓扑异构酶I突变体在DNA促旋酶基因中存在补偿性突变。
Cell. 1982 Nov;31(1):43-51. doi: 10.1016/0092-8674(82)90403-2.
6
Escherichia coli DNA topoisomerase I mutants: increased supercoiling is corrected by mutations near gyrase genes.大肠杆菌DNA拓扑异构酶I突变体：超螺旋增加可通过gyrase基因附近的突变得到纠正。
Cell. 1982 Nov;31(1):35-42. doi: 10.1016/0092-8674(82)90402-0.
7
[DNA supercoiling and topoisomerases in Escherichia coli].[大肠杆菌中的DNA超螺旋与拓扑异构酶]
Rev Latinoam Microbiol. 1995 Jul-Sep;37(3):291-304.
8
A topoisomerase from Escherichia coli related to DNA gyrase.一种来自大肠杆菌的与DNA促旋酶相关的拓扑异构酶。
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6110-4. doi: 10.1073/pnas.76.12.6110.
9
Escherichia coli DNA gyrase: genetic analysis of gyrA and gyrB mutations responsible for thermosensitive enzyme activity.大肠杆菌DNA回旋酶：对导致温度敏感酶活性的gyrA和gyrB突变的遗传分析。
FEBS Lett. 1992 Nov 2;312(1):61-5. doi: 10.1016/0014-5793(92)81410-n.
10
Expression in Escherichia coli of Y5 mutant and N-terminal domain-deleted DNA gyrase B proteins affects strongly plasmid maintenance.Y5突变体和N端结构域缺失的DNA促旋酶B蛋白在大肠杆菌中的表达强烈影响质粒的维持。
Plasmid. 1998;39(1):21-34. doi: 10.1006/plas.1997.1322.

引用本文的文献

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Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9766-71. doi: 10.1073/pnas.171579898. Epub 2001 Aug 7.
2
Impact of gyrA and parC mutations on quinolone resistance, doubling time, and supercoiling degree of Escherichia coli.gyrA和parC突变对大肠杆菌喹诺酮耐药性、倍增时间和超螺旋程度的影响。
Antimicrob Agents Chemother. 1999 Apr;43(4):868-75. doi: 10.1128/AAC.43.4.868.
3
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱，第10版：传统图谱。
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
4
Sequence specificity of illegitimate plasmid recombination in Bacillus subtilis: possible recognition sites for DNA topoisomerase I.枯草芽孢杆菌中非法质粒重组的序列特异性：DNA拓扑异构酶I的可能识别位点。
Nucleic Acids Res. 1998 May 15;26(10):2366-73. doi: 10.1093/nar/26.10.2366.
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Characterization of fluoroquinolone-resistant mutants of escherichia coli selected in vitro.体外筛选的大肠杆菌氟喹诺酮耐药突变体的特性分析
Antimicrob Agents Chemother. 1994 Jun;38(6):1284-91. doi: 10.1128/AAC.38.6.1284.
6
Structure and function of type II DNA topoisomerases.II型DNA拓扑异构酶的结构与功能。
Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):681-95. doi: 10.1042/bj3030681.
7
Biochemistry of homologous recombination in Escherichia coli.大肠杆菌中同源重组的生物化学
Microbiol Rev. 1994 Sep;58(3):401-65. doi: 10.1128/mr.58.3.401-465.1994.
8
Functions of the gene products of Escherichia coli.大肠杆菌基因产物的功能。
Microbiol Rev. 1993 Dec;57(4):862-952. doi: 10.1128/mr.57.4.862-952.1993.

本文引用的文献

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Regulation of the genes for E. coli DNA gyrase: homeostatic control of DNA supercoiling.大肠杆菌DNA促旋酶基因的调控：DNA超螺旋的稳态控制
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Regulation of DNA supercoiling in Escherichia coli.大肠杆菌中DNA超螺旋的调控
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Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes.大肠杆菌DNA拓扑异构酶I突变体在DNA促旋酶基因中存在补偿性突变。
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Escherichia coli DNA topoisomerase I mutants: increased supercoiling is corrected by mutations near gyrase genes.大肠杆菌DNA拓扑异构酶I突变体：超螺旋增加可通过gyrase基因附近的突变得到纠正。
Cell. 1982 Nov;31(1):35-42. doi: 10.1016/0092-8674(82)90402-0.
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DNA gyrase action involves the introduction of transient double-strand breaks into DNA.DNA 回旋酶的作用涉及在 DNA 中引入瞬时双链断裂。
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Replication and repair of DNA in cells of Escherichia coli treated with toluene.用甲苯处理的大肠杆菌细胞中DNA的复制与修复
Proc Natl Acad Sci U S A. 1970 Oct;67(2):674-81. doi: 10.1073/pnas.67.2.674.
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Mapping the active site tyrosine of Escherichia coli DNA gyrase.绘制大肠杆菌DNA促旋酶的活性位点酪氨酸图谱。
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DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy.大肠杆菌gyrB基因的DNA序列：一种新测序策略的应用
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Topoisomerase I mutants: the gene on pBR322 that encodes resistance to tetracycline affects plasmid DNA supercoiling.拓扑异构酶I突变体：pBR322上编码对四环素抗性的基因影响质粒DNA超螺旋。
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DNA topoisomerases.DNA拓扑异构酶
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