Gorga F, Ianniello R, Petrillo G, Provenzi E, Tufano M A
Istituto di Microbiologia, I Facoltà di Medicina e Chirurgia, Università di Napoli, Italy.
Microbiologica. 1992 Apr;15(2):187-90.
In previous works, we noted that porins, surface components of Gram negative bacteria, are toxic for human spermatozoa. In this work we see that porins of E. coli labeled with I125 (0.1-1.0 microgram/ml) bind to spermatozoa, showing the presence of saturable molecular configurations of 0.5-0.6 micrograms/ml. In the presence of non labeled porins, the binding of labeled porins to the spermatozoa is greatly reduced. At saturating concentrations of 0.6 micrograms/ml labeled porins, the binding reaches a saturation plateau at about 15 min.
在之前的研究中，我们注意到孔蛋白，革兰氏阴性菌的表面成分，对人类精子有毒性。在这项研究中，我们发现用碘125标记的大肠杆菌孔蛋白（0.1 - 1.0微克/毫升）与精子结合，显示出存在饱和度为0.5 - 0.6微克/毫升的分子构型。在未标记孔蛋白存在的情况下，标记孔蛋白与精子的结合显著减少。在标记孔蛋白浓度达到饱和的0.6微克/毫升时，结合在大约15分钟时达到饱和平台期。
