Sturm E, Bontrop R E, Vreugdenhil R J, Otting N, Bolhuis R L
Department of Immunology, Dr. Daniel den Hoed Cancer Center, Rotterdam, The Netherlands.
Immunogenetics. 1992;36(5):294-301. doi: 10.1007/BF00215657.
The human and chimpanzee T-cell receptor gamma-delta (TCR gamma delta) bearing cells represent a minor subset (3-8%) of T lymphocytes. In the periphery, the TCR gamma delta population has a restricted combinatorial repertoire. The TCRD-V1 and -V2 gene products are expressed in a mutually exclusive fashion, whereas, the TCRD-V2 and the TCRG-V9 encoded proteins show, in general, a coordinated expression. Restriction fragment length polymorphism analysis showed conservation of the restriction sites that identify the TCRG-V9 and TCRD-V2 rearrangements. The human TCRG-V9 locus has two alleles, TCRG-V9A1 and TCRG-V9A2 differing at codon position 31. The chimpanzee TCRG-V9 gene product differs from the products of the human TCRG-V9A1 and TCRG-V9A2 allele by two and three amino acid replacements, respectively. The human and the chimpanzee TCRG-V9-TCRD-V2 lymphocytes show a similar specific proliferative and cytolytic response to human Daudi Burkitt's lymphoma cells. Therefore, the amino acid replacements found in the chimpanzee TCRG-V9 gene product do not change the superantigen specificity across this species barrier.
携带人类和黑猩猩T细胞受体γ-δ（TCRγδ）的细胞占T淋巴细胞的一小部分（3-8%）。在外周血中，TCRγδ细胞群体具有有限的组合库。TCRD-V1和-V2基因产物以互斥的方式表达，而TCRD-V2和TCRG-V9编码的蛋白质通常表现出协同表达。限制性片段长度多态性分析表明，识别TCRG-V9和TCRD-V2重排的限制性位点具有保守性。人类TCRG-V9基因座有两个等位基因，TCRG-V9A1和TCRG-V9A2，在密码子位置31处不同。黑猩猩TCRG-V9基因产物与人类TCRG-V9A1和TCRG-V9A2等位基因产物分别有两个和三个氨基酸替换。人类和黑猩猩的TCRG-V9-TCRD-V2淋巴细胞对人类Daudi伯基特淋巴瘤细胞表现出相似的特异性增殖和细胞溶解反应。因此，在黑猩猩TCRG-V9基因产物中发现的氨基酸替换不会改变跨物种屏障的超抗原特异性。
