Morgello S
Department of Pathology (Neuropathology), Mount Sinai Medical Center, New York, New York 10029.
Am J Pathol. 1992 Aug;141(2):441-50.
The prevalence of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV) in acquired immunodeficiency syndrome (AIDS)-related primary central nervous system (CNS) lymphoma was examined. Deoxyribonucleic acid (DNA) extracted from 12 formalin-fixed, paraffin-embedded tumors was used as substrate for the polymerase chain reaction (PCR). Targets for amplification were the EBNA-1 region of EBV, the gag region of HIV, and a single copy cellular sequence as a control. The cases studied were autopsy and surgical specimens collected between the years 1985 and 1989. By the working formulation for non-Hodgkin's lymphomas, five had large cell, four had mixed large and small cleaved cell, two had small cleaved cell, and one had an unclassified histology. Epstein-Barr virus was detected in 6 of 12 tumors studied. Human immunodeficiency virus was not detected in any of the tumors. The presence of EBV was not correlated with any particular histologic tumor type. It is concluded that EBV, not HIV, can be detected in a large percentage (50%) of AIDS-related primary central nervous system (CNS) lymphomas. This viral association may be significant in light of the demonstrated ability of EBV to induce lymphoid tumors in experimental mammalian systems.
对爱泼斯坦-巴尔病毒（EBV）和人类免疫缺陷病毒（HIV）在获得性免疫缺陷综合征（AIDS）相关的原发性中枢神经系统（CNS）淋巴瘤中的流行情况进行了研究。从12个福尔马林固定、石蜡包埋的肿瘤中提取的脱氧核糖核酸（DNA）用作聚合酶链反应（PCR）的模板。扩增的靶标是EBV的EBNA-1区域、HIV的gag区域以及一个单拷贝细胞序列作为对照。所研究的病例为1985年至1989年间收集的尸检和手术标本。根据非霍奇金淋巴瘤的工作分类法，5例为大细胞型，4例为大细胞和小裂细胞混合型，2例为小裂细胞型，1例组织学类型未分类。在12个研究的肿瘤中，6个检测到爱泼斯坦-巴尔病毒。在任何肿瘤中均未检测到人类免疫缺陷病毒。EBV的存在与任何特定的组织学肿瘤类型均无相关性。得出的结论是，在很大比例（50%）的AIDS相关的原发性中枢神经系统（CNS）淋巴瘤中可检测到EBV，而非HIV。鉴于EBV在实验性哺乳动物系统中具有诱导淋巴瘤的能力，这种病毒关联可能具有重要意义。
