Gallo D, Hoffman M N, Yeh E T, George J R, Hanson C V
Viral and Rickettsial Disease Laboratory, California Department of Health Services, Berkeley 94704.
J Clin Microbiol. 1992 Sep;30(9):2275-8. doi: 10.1128/jcm.30.9.2275-2278.1992.
Serum samples from 20 human immunodeficiency virus type 1 (HIV-1)- and 30 HIV-2-infected and 7 dually infected individuals were reacted by using the indirect immunofluorescence assay (IFA) and membrane fluorescence assay in order to determine whether these methods were useful for typing HIV-1 and HIV-2 antibodies. Although 41 of 50 (82%) of the HIV-1- and HIV-2-positive specimens cross-reacted to some extent with the heterologous antigen in the IFA, the antigen with the higher titer correlated completely with the infecting type. The IFA could not distinguish single from dual infections, however. In contrast, only 4 of the 50 (8%) serum samples cross-reacted in the membrane fluorescence test. All seven of the specimens from patients with mixed infections reacted with both antigens. The membrane fluorescence test appears to be reliable for serodifferentiation of HIV-1 and HIV-2 infections and may be useful for laboratories with low-volume typing requirements.
为了确定间接免疫荧光法（IFA）和膜荧光法是否可用于HIV-1和HIV-2抗体分型，对20例1型人类免疫缺陷病毒（HIV-1）感染者、30例HIV-2感染者及7例双重感染者的血清样本进行了检测。尽管在IFA中，50份HIV-1和HIV-2阳性标本中有41份（82%）与异源抗原存在一定程度的交叉反应，但滴度较高的抗原与感染类型完全相符。然而，IFA无法区分单一感染和双重感染。相比之下，在膜荧光试验中，50份血清样本中仅有4份（8%）发生交叉反应。所有7份混合感染患者的标本均与两种抗原发生反应。膜荧光试验似乎可可靠地用于HIV-1和HIV-2感染的血清学鉴别，对于检测量较小的分型需求实验室可能有用。
