一种新型成肌细胞增强子元件介导MyoD转录。
A novel myoblast enhancer element mediates MyoD transcription.
作者信息
Tapscott S J, Lassar A B, Weintraub H
机构信息
Fred Hutchinson Cancer Research Center, Howard Hughes Medical Institute Laboratory, Seattle, Washington 98104.
出版信息
Mol Cell Biol. 1992 Nov;12(11):4994-5003. doi: 10.1128/mcb.12.11.4994-5003.1992.
Abstract
The MyoD gene can orchestrate the expression of the skeletal muscle differentiation program. We have identified the regions of the gene necessary to reproduce transcription specific to skeletal myoblasts and myotubes. A proximal regulatory region (PRR) contains a conserved TATA box, a CCAAT box, and a GC-rich region that includes a consensus SP1 binding site. The PRR is sufficient for high levels of skeletal muscle-specific activity in avian muscle cells. In murine cells the PRR alone has only low levels of activity and requires an additional distal regulatory region to achieve high levels of muscle-specific activity. The distal regulatory region differs from a conventional enhancer in that chromosomal integration appears necessary for productive interactions with the PRR. While the Moloney leukemia virus long terminal repeat can enhance transcription from the MyoD PRR in both transient and stable assays, the simian virus 40 enhancer cannot, suggesting that specific enhancer-promoter interactions are necessary for PRR function.
摘要
肌细胞生成素基因（MyoD基因）能够协调骨骼肌分化程序的表达。我们已经确定了该基因中重现骨骼肌成肌细胞和肌管特异性转录所必需的区域。一个近端调控区域（PRR）包含一个保守的TATA框、一个CCAAT框以及一个富含GC的区域，该区域包含一个共有SP1结合位点。PRR足以在禽类肌肉细胞中实现高水平的骨骼肌特异性活性。在鼠细胞中，单独的PRR只有低水平的活性，需要一个额外的远端调控区域才能实现高水平的肌肉特异性活性。远端调控区域与传统增强子的不同之处在于，染色体整合似乎是与PRR进行有效相互作用所必需的。虽然莫洛尼白血病病毒长末端重复序列在瞬时和稳定试验中都能增强MyoD PRR的转录，但猿猴病毒40增强子却不能，这表明PRR功能需要特定的增强子-启动子相互作用。
相似文献
1
A novel myoblast enhancer element mediates MyoD transcription.一种新型成肌细胞增强子元件介导MyoD转录。
Mol Cell Biol. 1992 Nov;12(11):4994-5003. doi: 10.1128/mcb.12.11.4994-5003.1992.
2
A novel myogenic regulatory circuit controls slow/cardiac troponin C gene transcription in skeletal muscle.一种新型的生肌调节回路控制骨骼肌中慢肌/心肌肌钙蛋白C基因的转录。
Mol Cell Biol. 1994 Mar;14(3):1870-85. doi: 10.1128/mcb.14.3.1870-1885.1994.
3
A novel site, Mt, in the human desmin enhancer is necessary for maximal expression in skeletal muscle.人结蛋白增强子中的一个新位点Mt对于在骨骼肌中的最大表达是必需的。
J Biol Chem. 1998 Mar 13;273(11):6402-9. doi: 10.1074/jbc.273.11.6402.
4
5
A single MEF-2 site is a major positive regulatory element required for transcription of the muscle-specific subunit of the human phosphoglycerate mutase gene in skeletal and cardiac muscle cells.单个MEF-2位点是人类磷酸甘油酸变位酶基因的肌肉特异性亚基在骨骼肌和心肌细胞中转录所需的主要正调控元件。
Mol Cell Biol. 1992 Oct;12(10):4384-90. doi: 10.1128/mcb.12.10.4384-4390.1992.
6
A new myocyte-specific enhancer-binding factor that recognizes a conserved element associated with multiple muscle-specific genes.一种新的心肌细胞特异性增强子结合因子，它识别与多个肌肉特异性基因相关的保守元件。
Mol Cell Biol. 1989 Nov;9(11):5022-33. doi: 10.1128/mcb.9.11.5022-5033.1989.
7
Regulatory elements that control the lineage-specific expression of myoD.
Science. 1992 Apr 24;256(5056):538-42. doi: 10.1126/science.1315077.
8
A skeletal muscle-specific enhancer regulated by factors binding to E and CArG boxes is present in the promoter of the mouse myosin light-chain 1A gene.小鼠肌球蛋白轻链1A基因启动子中存在一个由与E盒和CArG盒结合的因子调控的骨骼肌特异性增强子。
Mol Cell Biol. 1995 Aug;15(8):4585-96. doi: 10.1128/MCB.15.8.4585.
9
Cloning of Rous sarcoma virus enhancer factor genes. II. RSV-EF-II, abundantly expressed in fibroblasts and muscle tissue, binds to an octamer sequence, 5'-GTACCACC-3', in the noncoding strand of RSV enhancer.劳氏肉瘤病毒增强子因子基因的克隆。II. RSV-EF-II在成纤维细胞和肌肉组织中大量表达，与劳氏肉瘤病毒增强子非编码链上的八聚体序列5'-GTACCACC-3'结合。
Virology. 1996 Aug 1;222(1):133-43. doi: 10.1006/viro.1996.0404.
10
Molecular cloning and functional analysis of the promoter of rat skeletal muscle voltage-sensitive sodium channel subtype 2 (rSkM2): evidence for muscle-specific nuclear protein binding to the core promoter.大鼠骨骼肌电压敏感性钠通道亚型2（rSkM2）启动子的分子克隆与功能分析：肌肉特异性核蛋白与核心启动子结合的证据
DNA Cell Biol. 1994 Jan;13(1):9-23. doi: 10.1089/dna.1994.13.9.
引用本文的文献
1
Augmenting and directing long-range CRISPR-mediated activation in human cells.增强和定向人类细胞中长距离 CRISPR 介导的激活
Nat Methods. 2021 Sep;18(9):1075-1081. doi: 10.1038/s41592-021-01224-1. Epub 2021 Aug 5.
2
The SNPs in myoD gene from normal muscle developing individuals have no effect on muscle mass.正常肌肉发育个体的 myoD 基因中的 SNPs 对肌肉质量没有影响。
BMC Genet. 2019 Sep 2;20(1):72. doi: 10.1186/s12863-019-0772-6.
3
Master control: transcriptional regulation of mammalian Myod.主控：哺乳类 Myod 的转录调控。
J Muscle Res Cell Motil. 2019 Jun;40(2):211-226. doi: 10.1007/s10974-019-09538-6. Epub 2019 Jul 12.
4
MUNC, an Enhancer RNA Upstream from the Gene, Induces a Subgroup of Myogenic Transcripts in Independently of MyoD.MUNC，位于 基因上游的增强子 RNA，可在不依赖 MyoD 的情况下独立诱导一组肌源性转录本。
Mol Cell Biol. 2018 Sep 28;38(20). doi: 10.1128/MCB.00655-17. Print 2018 Oct 15.
5
A Muscle-Specific Enhancer RNA Mediates Cohesin Recruitment and Regulates Transcription In trans.肌特异性增强子 RNA 介导黏着蛋白募集并调节反式转录。
Mol Cell. 2018 Jul 5;71(1):129-141.e8. doi: 10.1016/j.molcel.2018.06.008.
6
Association of lamin A/C with muscle gene-specific promoters in myoblasts.成肌细胞中核纤层蛋白A/C与肌肉基因特异性启动子的关联。
Biochem Biophys Rep. 2015 Aug 29;4:76-82. doi: 10.1016/j.bbrep.2015.08.021. eCollection 2015 Dec.
7
MyoD- and FoxO3-mediated hotspot interaction orchestrates super-enhancer activity during myogenic differentiation.MyoD和FoxO3介导的热点相互作用在成肌分化过程中协调超级增强子活性。
Nucleic Acids Res. 2017 Sep 6;45(15):8785-8805. doi: 10.1093/nar/gkx488.
8
Deltex2 represses MyoD expression and inhibits myogenic differentiation by acting as a negative regulator of Jmjd1c.Deltex2 通过作为 Jmjd1c 的负调节剂来抑制 MyoD 表达并抑制成肌分化。
Proc Natl Acad Sci U S A. 2017 Apr 11;114(15):E3071-E3080. doi: 10.1073/pnas.1613592114. Epub 2017 Mar 28.
9
Expression of myogenic regulatory factors in chicken embryos during somite and limb development.体节和肢体发育过程中鸡胚成肌调节因子的表达
J Anat. 2015 Sep;227(3):352-60. doi: 10.1111/joa.12340. Epub 2015 Jul 16.
10
Tissue-specific epigenetics in gene neighborhoods: myogenic transcription factor genes.基因邻域中的组织特异性表观遗传学：成肌转录因子基因
Hum Mol Genet. 2015 Aug 15;24(16):4660-73. doi: 10.1093/hmg/ddv198. Epub 2015 Jun 3.
本文引用的文献
1
Cytoplasmic activation of human nuclear genes in stable heterocaryons.稳定异核体中人类核基因的细胞质激活
Cell. 1983 Apr;32(4):1171-80. doi: 10.1016/0092-8674(83)90300-8.
2
Expression of a single transfected cDNA converts fibroblasts to myoblasts.单个转染的互补脱氧核糖核酸（cDNA）的表达可将成纤维细胞转化为成肌细胞。
Cell. 1987 Dec 24;51(6):987-1000. doi: 10.1016/0092-8674(87)90585-x.
3
A multiplicity of CCAAT box-binding proteins.多种CCAAT框结合蛋白。
Cell. 1987 Sep 11;50(6):863-72. doi: 10.1016/0092-8674(87)90513-7.
4
A nonchromatographic assay for expression of the chloramphenicol acetyltransferase gene in eucaryotic cells.一种用于检测真核细胞中氯霉素乙酰转移酶基因表达的非色谱分析方法。
Anal Biochem. 1986 Jul;156(1):251-6. doi: 10.1016/0003-2697(86)90180-6.
5
A conserved CATTCCT motif is required for skeletal muscle-specific activity of the cardiac troponin T gene promoter.心脏肌钙蛋白T基因启动子的骨骼肌特异性活性需要一个保守的CATTCCT基序。
Proc Natl Acad Sci U S A. 1988 Sep;85(17):6404-8. doi: 10.1073/pnas.85.17.6404.
6
Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses.反式作用蛋白因子与真核转录调控：来自DNA肿瘤病毒研究的经验教训
Genes Dev. 1988 Mar;2(3):267-81. doi: 10.1101/gad.2.3.267.
7
Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD.通过强制表达MyoD在色素、神经、脂肪、肝脏和成纤维细胞系中激活肌肉特异性基因。
Proc Natl Acad Sci U S A. 1989 Jul;86(14):5434-8. doi: 10.1073/pnas.86.14.5434.
8
A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts.一种与MyoD1相关但又不同的新型人类肌肉因子可诱导10T1/2成纤维细胞发生肌源性转化。
EMBO J. 1989 Mar;8(3):701-9. doi: 10.1002/j.1460-2075.1989.tb03429.x.
9
A new myocyte-specific enhancer-binding factor that recognizes a conserved element associated with multiple muscle-specific genes.一种新的心肌细胞特异性增强子结合因子，它识别与多个肌肉特异性基因相关的保守元件。
Mol Cell Biol. 1989 Nov;9(11):5022-33. doi: 10.1128/mcb.9.11.5022-5033.1989.
10
Differential expression of myogenic determination genes in muscle cells: possible autoactivation by the Myf gene products.肌源性决定基因在肌肉细胞中的差异表达：Myf基因产物可能的自激活作用。
EMBO J. 1989 Dec 1;8(12):3617-25. doi: 10.1002/j.1460-2075.1989.tb08535.x.
Zotero 插件