传染性胃肠炎冠状病毒：强毒株和弱毒株诱导产生的表面抗原
Transmissible gastroenteritis coronavirus: surface antigens induced by virulent and attenuated strains.
作者信息
To L T, Bernard S, Bottreau E
机构信息
Institute of Veterinary Research, Laboratory of Virology, Bach mai-Hànôi, Viêtnam.
出版信息
Res Virol. 1992 Jul-Aug;143(4):241-8. doi: 10.1016/s0923-2516(06)80112-3.
Abstract
Three strains of the transmissible gastroenteritis virus (TGEV) possessing different degrees of pathogenicity for piglets were examined for their capacity to express M and S glycoproteins on the infected cell surface using a microwell immunoperoxidase test. These two viral glycoproteins were easily detected on the plasma membrane of 0.1% paraformaldehyde-fixed swine testis (ST) or pig kidney (RP.D) cells which were infected with high-passaged Purdue-115 and low-passaged D-52 strains and a high-passaged attenuated (188-SG) mutant of TGEV. No significant differences were found between attenuated and virulent strains with regard to the viral antigen expression on the membrane of infected cells over a 14-h period.
摘要
利用微孔免疫过氧化物酶试验，检测了三株对仔猪具有不同致病程度的传染性胃肠炎病毒（TGEV）在感染细胞表面表达M和S糖蛋白的能力。在感染了高传代的普渡-115株、低传代的D-52株以及TGEV的高传代减毒（188-SG）突变株的0.1%多聚甲醛固定的猪睾丸（ST）或猪肾（RP.D）细胞的质膜上，很容易检测到这两种病毒糖蛋白。在14小时的时间段内，减毒株和强毒株在感染细胞膜上的病毒抗原表达方面未发现显著差异。
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