Modalsli K, Bukholm G, Degré M
Kaptein W. Wilhelmsen, Frues Bakteriologiske Institutt, University of Oslo, Norway.
J Biol Regul Homeost Agents. 1992 Apr-Jun;6(2):35-45.
The effect of interferon treatment on interaction of Shigella flexneri with in vitro cultured cells was investigated. Pretreatment of HEp-2 cells with human interferons had no effect on the susceptibility of cells to S. flexneri, measured by invasiveness and adhesiveness. Human leukocyte interferon and human recombinant interferon-alpha-A reduced adhesiveness, intracellular multiplication and invasiveness of S. flexneri in HEp-2 cells preinfected with coxsackie B1 virus. Also non-receptor mediated-phagocytosis was reduced by interferon treatment in virus infected cells. The interferon effects were dependent on continuous protein synthesis, because they were not expressed when cycloheximide or abrin was added to the virus infected cell cultures. No effect of interferon was detected on intracellular content of Na+ or K+, Na(+)-K+ activated ATPase activity or cytoplasma membrane polarity, in virus infected or control cell cultures. The interferon effect on bacterial invasiveness seems to be dependent on an interferon receptor interaction on cytoplasma membrane level because directly microinjected interferon showed no effect.
研究了干扰素治疗对福氏志贺菌与体外培养细胞相互作用的影响。用人干扰素预处理HEp - 2细胞，通过侵袭性和黏附性测量，对细胞对福氏志贺菌的易感性没有影响。人白细胞干扰素和人重组干扰素 - α - A降低了在柯萨奇B1病毒预感染的HEp - 2细胞中福氏志贺菌的黏附性、细胞内增殖和侵袭性。在病毒感染的细胞中，干扰素治疗也降低了非受体介导的吞噬作用。干扰素的作用依赖于持续的蛋白质合成，因为当向病毒感染的细胞培养物中加入环己酰亚胺或相思豆毒素时，它们不表达。在病毒感染或对照细胞培养物中，未检测到干扰素对Na⁺或K⁺的细胞内含量、Na⁺ - K⁺激活的ATP酶活性或细胞质膜极性有影响。干扰素对细菌侵袭性的作用似乎依赖于细胞质膜水平上的干扰素受体相互作用，因为直接显微注射的干扰素没有效果。
