Hilton D A, Fletcher A, Pringle J H
Department of Neuropathology, Frenchay Hospital, Bristol, UK.
Neuropathol Appl Neurobiol. 1994 Jun;20(3):238-42. doi: 10.1111/j.1365-2990.1994.tb00965.x.
The role of coxsackie virus infection in the pathogenesis of idiopathic inflammatory muscle disease (IIMD) has been investigated by many workers with conflicting results. This study uses in situ hybridization, with digoxigenin-labelled oligonucleotide probes complementary to the coxsackie B virus genome, to investigate the presence of virus RNA in muscle biopsies from 26 patients with IIMD. In the five cases of inclusion body myositis studied, there was focal probe-binding to nuclei and cytoplasm, and in nine cases probe-binding to mast cells was seen. In both of these instances probe-binding was non-specific and not due to hybridization. None of the cases showed the presence of coxsackie virus RNA within muscle and it is concluded that lytic infection of myocytes by coxsackie virus does not occur in IIMD.
许多研究人员对柯萨奇病毒感染在特发性炎性肌病（IIMD）发病机制中的作用进行了研究，但结果相互矛盾。本研究采用地高辛标记的与柯萨奇B病毒基因组互补的寡核苷酸探针进行原位杂交，以调查26例IIMD患者肌肉活检中病毒RNA的存在情况。在所研究的5例包涵体肌炎病例中，有局灶性探针与细胞核和细胞质结合，在9例病例中可见探针与肥大细胞结合。在这两种情况下，探针结合均为非特异性的，并非由杂交引起。所有病例均未显示肌肉内存在柯萨奇病毒RNA，因此得出结论，IIMD中不存在柯萨奇病毒对肌细胞的溶细胞感染。
