直接从临床标本原代培养物中快速测定人巨细胞病毒对更昔洛韦的敏感性。
Rapid determination of human cytomegalovirus susceptibility to ganciclovir directly from clinical specimen primocultures.
作者信息
Pepin J M, Simon F, Dussault A, Collin G, Dazza M C, Brun-Vezinet F
机构信息
Department of Virology, Claude Bernard Hospital, Paris, France.
出版信息
J Clin Microbiol. 1992 Nov;30(11):2917-20. doi: 10.1128/jcm.30.11.2917-2920.1992.
Abstract
We were able to rapidly determine the susceptibility of human cytomegalovirus to ganciclovir using a late antigen synthesis reduction assay directly on primocultures of clinical specimens. This test was compared with a conventional susceptibility assay which was performed with cell-free virus obtained after cytomegalovirus isolation and in vitro passages. Both tests produced similar results. The rapid test, unlike conventional assays, is able to provide a result within 5 days after receipt of the specimen and could thus play a direct role in the therapeutic decision.
摘要
我们能够使用晚期抗原合成减少试验，直接对临床标本的原代培养物快速测定人巨细胞病毒对更昔洛韦的敏感性。将该试验与传统的敏感性试验进行比较，传统试验是用巨细胞病毒分离和体外传代后获得的无细胞病毒进行的。两种试验产生了相似的结果。与传统试验不同，快速试验能够在收到标本后5天内给出结果，因此可以在治疗决策中发挥直接作用。
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