S-亚硝基谷胱甘肽在体内外均能抑制血小板活化。
S-nitroso-glutathione inhibits platelet activation in vitro and in vivo.
作者信息
Radomski M W, Rees D D, Dutra A, Moncada S
机构信息
Wellcome Research Laboratories, Beckenham, Kent.
出版信息
Br J Pharmacol. 1992 Nov;107(3):745-9. doi: 10.1111/j.1476-5381.1992.tb14517.x.
Abstract
- The effect of S-nitroso-glutathione (GSNO), a stable nitrosothiol, on platelet activation was examined in vitro and in vivo. 2. The adhesion of human platelets to fibrillar collagen and human endothelial cell monolayers was inhibited by GSNO. 3. GSNO caused a concentration-dependent inhibition of collagen-induced platelet aggregation in vitro and decreased ADP-induced aggregation in the conscious rat. 4. Inhibition of platelet aggregation in vitro correlated with the increase in intraplatelet cyclic GMP levels. 5. The release of NO from GSNO was enhanced by platelet lysate, native glutathione and ascorbate. 6. The results show that GSNO is a carrier of NO and therefore has pharmacological activity as an inhibitor of platelet activation.
摘要
- 研究了稳定的亚硝基硫醇S-亚硝基谷胱甘肽（GSNO）对血小板活化的体内外作用。2. GSNO抑制人血小板与纤维状胶原蛋白及人内皮细胞单层的黏附。3. GSNO在体外对胶原蛋白诱导的血小板聚集产生浓度依赖性抑制，并降低清醒大鼠中ADP诱导的聚集。4. 体外血小板聚集的抑制与血小板内环磷酸鸟苷水平的升高相关。5. 血小板裂解物、天然谷胱甘肽和抗坏血酸可增强GSNO中NO的释放。6. 结果表明，GSNO是NO的载体，因此作为血小板活化抑制剂具有药理活性。
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