采用聚合酶链反应（PCR）检测肾移植受者血清中的巨细胞病毒。
Detection of cytomegalovirus using PCR in serum from renal transplant recipients.
作者信息
Cunningham R, Harris A, Frankton A, Irving W
机构信息
Department of Microbiology, University Hospital, Queen's Medical Centre, Nottingham.
出版信息
J Clin Pathol. 1995 Jun;48(6):575-7. doi: 10.1136/jcp.48.6.575.
AIMS
To develop a polymerase chain reaction (PCR) assay for the detection of cytomegalovirus (CMV) DNA in serum and leucocytes of renal transplant recipients and compare this assay with CMV culture and serodiagnosis.
METHODS
Monthly specimens were obtained from 12 patients starting immediately before transplant. CMV infection was monitored by IgM enzyme linked immunosorbent assay, virus culture and PCR on serum and leucocytes.
RESULTS
Two of four IgG positive patients had reactivation of CMV disease confirmed by culture, three of eight seronegative patients had a primary infection, one confirmed by serology and two by culture. PCR was positive earlier than conventional methods in three cases and concurrently in two. No positive PCR reactions occurred in the seven patients who remained negative by culture and serology.
CONCLUSIONS
CMV DNA is detectable in serum; serum may be positive before virus is detectable by buffy coat culture; and PCR may be useful as an early indication of potential CMV disease in renal transplant recipients.
目的
开发一种聚合酶链反应（PCR）检测方法，用于检测肾移植受者血清和白细胞中的巨细胞病毒（CMV）DNA，并将该检测方法与CMV培养和血清学诊断进行比较。
方法
从12例患者移植前即刻开始每月采集标本。通过IgM酶联免疫吸附试验、病毒培养以及血清和白细胞的PCR对CMV感染进行监测。
结果
4例IgG阳性患者中有2例经培养证实CMV疾病复发，8例血清阴性患者中有3例发生原发性感染，1例经血清学证实，2例经培养证实。PCR在3例中比传统方法更早呈阳性，在2例中同时呈阳性。7例培养和血清学均为阴性的患者未出现PCR阳性反应。
结论
血清中可检测到CMV DNA；在通过血沉棕黄层培养可检测到病毒之前血清可能呈阳性；PCR可能有助于作为肾移植受者潜在CMV疾病的早期指标。
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