Mackiewicz S, Mackiewicz U, Burchardt K, Konys J
Z Immunitatsforsch Exp Klin Immunol. 1976 Mar;151(1-2):13-21.
Young adult CFW mice, injected with sheep erythrocytes, were treated with cyclophosphamide and sacrificed at different times. The activity of acid phosphatase and for non-specific esterase was measured by semi-quantitative methods in smears prepared from cell suspension of draining lymph node. In cyclophosphamide-treated mice was noted a significant decrease in acid phosphatase activity in lymphoid cells, expressed both as average activity in single lymphoid cell and a decrease in proportion of cells exhibiting enzyme activity. Also a short-term decrease was observed in activity of non-specific esterase caused both by disappearance of highly active large lymphoid and blast cells as well as decrease in percentage of enzyme-positive small lymphocytes.
给年轻成年CFW小鼠注射绵羊红细胞后，用环磷酰胺进行处理，并在不同时间处死。通过半定量方法在由引流淋巴结细胞悬体制备的涂片上测定酸性磷酸酶和非特异性酯酶的活性。在环磷酰胺处理的小鼠中，观察到淋巴样细胞中酸性磷酸酶活性显著降低，这既表现为单个淋巴样细胞的平均活性降低，也表现为显示酶活性的细胞比例下降。还观察到非特异性酯酶活性短期降低，这是由于高活性大淋巴样细胞和母细胞消失以及酶阳性小淋巴细胞百分比降低所致。
