利用重组自交系和随机扩增多态性DNA对拟南芥进行全基因组和局部基因组图谱绘制。

Reiter R S, Williams J G, Feldmann K A, Rafalski J A, Tingey S V, Scolnik P A

E.I Du Pont de Nemours & Company, Experimental Station, Wilmington, DE 19880-0402.

Proc Natl Acad Sci U S A. 1992 Feb 15;89(4):1477-81. doi: 10.1073/pnas.89.4.1477.

A population of Arabidopsis thaliana recombinant inbred lines was constructed and used to develop a high-density genetic linkage map containing 252 random amplified polymorphic DNA markers and 60 previously mapped restriction fragment length polymorphisms. Linkage groups were correlated to the classical genetic map by inclusion of nine phenotypic markers in the mapping cross. We also applied a technique for local mapping that allows targeting of markers to a selected genome region by pooling DNA from recombinant inbred lines based on their genotype. We conclude that random amplified polymorphic DNAs, used in conjunction with a recombinant inbred population, can facilitate the genetic and physical characterization of the Arabidopsis genome and that this method is generally applicable to other organisms for which appropriate populations either are available or can be developed.

构建了拟南芥重组自交系群体，并用于构建一个高密度遗传连锁图谱，该图谱包含252个随机扩增多态性DNA标记和60个先前定位的限制性片段长度多态性标记。通过在作图杂交中纳入9个表型标记，将连锁群与经典遗传图谱相关联。我们还应用了一种局部作图技术，该技术通过根据重组自交系的基因型汇集DNA，将标记靶向选定的基因组区域。我们得出结论，随机扩增多态性DNA与重组自交群体结合使用，可以促进拟南芥基因组的遗传和物理特征分析，并且该方法通常适用于其他有合适群体可用或可开发的生物。