Claassen J L, Levine A D, Buckley R H
Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina 27710.
Cell Immunol. 1992 Apr;140(2):357-69. doi: 10.1016/0008-8749(92)90202-z.
Pokeweed mitogen (PWM) suppressed rhIL-4-induced IgE synthesis in a concentration-dependent manner. When rhIL-4 was present from Day 0, PWM added to cultures on Day 0 or 3 inhibited MNC IgE synthesis but not when it was added on Day 6 or later. The concentration of interferon-gamma (IFN-gamma) in MNC culture supernatants varied directly with the quantity of PWM added. Conversely, rhIL-4-stimulated MNC culture IgE concentrations varied inversely with the dose of PWM added and the IFN-gamma concentrations induced. The addition of a rabbit polyclonal neutralizing anti-human IFN-gamma antibody to rhIL-4 plus PWM-stimulated cultures partially or completely reversed PWM-induced inhibition of rhIL-4-induced IgE synthesis. PWM failed to inhibit rhIL-4-induced IgE synthesis by isolated B cells cocultured with monocytes and T cells from a clone unable to produce IFN-gamma message or protein. These findings are consistent with the postulate that PWM inhibits rhIL-4-induced IgE synthesis by inducing the production of IFN-gamma.
商陆丝裂原（PWM）以浓度依赖的方式抑制重组人白细胞介素-4（rhIL-4）诱导的IgE合成。当从第0天开始存在rhIL-4时，在第0天或第3天添加到培养物中的PWM可抑制单核细胞悬液（MNC）IgE合成，但在第6天或之后添加时则无此作用。MNC培养上清液中干扰素-γ（IFN-γ）的浓度与添加的PWM量直接相关。相反，rhIL-4刺激的MNC培养物IgE浓度与添加的PWM剂量和诱导的IFN-γ浓度呈负相关。向rhIL-4加PWM刺激的培养物中添加兔多克隆中和抗人IFN-γ抗体可部分或完全逆转PWM诱导的对rhIL-4诱导的IgE合成的抑制作用。PWM不能抑制与来自无法产生IFN-γ信使或蛋白质的克隆的单核细胞和T细胞共培养的分离B细胞的rhIL-4诱导的IgE合成。这些发现与PWM通过诱导IFN-γ的产生来抑制rhIL-4诱导的IgE合成这一假设一致。
