Tsuji T, Naito K, Tokuyama K, Okada T, Kosaka K
Acta Med Okayama. 1976 Feb;30(1):69-73.
HB surface antigen (HBs Ag) was detected using the enzyme-labelled antibody technique on routinely processed liver biopsy material fixed in Bouin's fixative and embedded in paraffin. Of 85 examined specimens, 45 cases were HBs Ag positive by both the immunofluorescent test and the enzyme labelled antibody technique. The remaining 40 cases were negative by both techniques. The specificity of HBs Ag detected by the enzyme-labelled antibody technique was confirmed by the blocking test using guinea pig specific HBs antibody. The results indicate that the enzyme-labelled antibody technique may be useful for detecting HBs Ag on routine paraffin sections.
采用酶标抗体技术，对用布因氏固定液固定并石蜡包埋的常规处理肝活检材料进行乙型肝炎表面抗原（HBs Ag）检测。在85份送检标本中，45例通过免疫荧光试验和酶标抗体技术均呈HBs Ag阳性。其余40例两种技术检测均为阴性。通过用豚鼠特异性HBs抗体进行阻断试验，证实了酶标抗体技术检测HBs Ag的特异性。结果表明，酶标抗体技术可用于在常规石蜡切片上检测HBs Ag。
