Sanchez V, Vazquez J A, Barth-Jones D, Dembry L, Sobel J D, Zervos M J
Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan 48202.
J Clin Microbiol. 1992 Nov;30(11):3005-8. doi: 10.1128/jcm.30.11.3005-3008.1992.
Candida species are important nosocomial pathogens; however, little is known about the epidemiology of Candida lusitaniae, an organism frequently resistant to amphotericin B. We evaluated 98 patients admitted to the bone marrow transplant and medical intensive care units of a tertiary-care hospital. Each patient with C. lusitaniae was matched with control patients. Restriction fragment analysis of DNA was used to determine strain relatedness. Seven patients (7.1%) with C. lusitaniae were identified; five acquired C. lusitaniae after admission to the study unit. All isolates were susceptible to amphotericin B. There were no differences between patients and controls with regard to duration of stay in the study unit, antibiotic administration, antifungal therapy, immunosuppressive therapy, catheter use, or underlying disease. Temporal and geographic clustering of five patients with identical strains occurred. No common source was identified. Restriction fragment analysis revealed a total of eight strains, and five patients shared one strain type. These results demonstrate exogenous acquisition of C. lusitaniae. The mechanism of acquisition is probably indirect contact transmission between patients.
念珠菌属是重要的医院感染病原体；然而，对于常常对两性霉素B耐药的葡萄牙念珠菌的流行病学情况却知之甚少。我们评估了一家三级医院骨髓移植科和内科重症监护病房收治的98例患者。每例感染葡萄牙念珠菌的患者均与对照患者进行匹配。采用DNA限制性片段分析来确定菌株的相关性。共识别出7例（7.1%）感染葡萄牙念珠菌的患者；其中5例在进入研究单位后感染了葡萄牙念珠菌。所有分离株均对两性霉素B敏感。在研究单位的住院时间、抗生素使用、抗真菌治疗、免疫抑制治疗、导管使用或基础疾病方面，患者与对照之间并无差异。5例携带相同菌株的患者出现了时间和地域上的聚集。未确定共同来源。限制性片段分析共揭示了8种菌株类型，5例患者共享一种菌株类型。这些结果表明葡萄牙念珠菌是外源性获得的。获得机制可能是患者之间的间接接触传播。
