MAGNUSSON M, GULD J, MAGNUS K, WAALER H
Bull World Health Organ. 1958;19(5):799-828.
Tuberculin is known to be adsorbed to containers and syringes. In the present paper, the adsorption which takes place in the ampoules has been studied in relation to the diluent for the tuberculin.Adsorption was most evident in dilutions prepared with saline or with phosphate buffer containing dextran. The inclusion in phosphate buffer diluent of small amounts of proteins or synthetic surface-active agents decreased or prevented adsorption. A boric-acid sodium-borate diluent containing gum arabic, previously recommended for the preparation of stabilized tuberculin dilutions, was found to be ineffective.The most suitable diluent for the preparation of stable tuberculin dilutions was a 0.05 per thousand solution of Tween 80 in phosphate-buffered saline; this diluent appeared to prevent adsorption under a variety of experimental conditions.The inclusion of Tween 80 in the diluent had little or no effect on the general storage stability of purified tuberculin.Sensitization experiments in guinea-pigs, rabbits and humans showed that no sensitization against Tween 80 need be feared when a 0.05 per thousand solution of Tween 80 in phosphate buffered saline is used in the preparation of tuberculin dilutions.
已知结核菌素会吸附在容器和注射器上。在本文中，针对结核菌素稀释剂，研究了安瓿中发生的吸附情况。在用生理盐水或含右旋糖酐的磷酸盐缓冲液制备的稀释液中，吸附最为明显。在磷酸盐缓冲液稀释剂中加入少量蛋白质或合成表面活性剂可减少或防止吸附。一种先前推荐用于制备稳定结核菌素稀释液、含阿拉伯胶的硼酸钠硼酸稀释剂，被发现无效。制备稳定结核菌素稀释液最合适的稀释剂是在磷酸盐缓冲盐水中含0.05‰吐温80的溶液；在各种实验条件下，这种稀释剂似乎都能防止吸附。在稀释剂中加入吐温80对纯化结核菌素的一般储存稳定性几乎没有影响。在豚鼠、兔子和人类身上进行的致敏实验表明，当在制备结核菌素稀释液时使用在磷酸盐缓冲盐水中含0.05‰吐温80的溶液时，无需担心对吐温80产生致敏反应。
