WAALER H, GULD J, MAGNUS K, MAGNUSSON M
Bull World Health Organ. 1958;19(5):783-98.
Dilutions of the purified tuberculin RT 19-21 (Statens Seruminstitut, Copenhagen) have been found to be subject to unsystematic variations in potency. Preliminary investigations suggested that adsorption of tuberculin on to the container might be the cause. This possibility has been further explored in a series of experiments on guinea-pigs that is reported in the present paper.IT IS DEMONSTRATED THAT TUBERCULIN IS, IN FACT, ADSORBED TO THE GLASS SURFACE OF THE CONTAINER: tuberculin-active eluates are obtained from ampoules previously used for tuberculin, with a buffer eluant containing Tween 80.Estimates indicate that a 5 TU dilution of RT 19-21 (prepared to contain 5 TU or 0.0001 mg per 0.1 ml) loses on an average 60% of its active substance through adsorption. The amount adsorbed differs from one ampoule to another, and a negative correlation is observed between the potency of the original contents of the ampoule and the potency of the eluate obtained when the ampoule is refilled with Tween 80 eluant.The extent of adsorption depends on the strength of the dilution, the temperature, and the duration and extent of contact between dilution and container. Another observation is that shaking of the ampoule reduces adsorption.There are indications that the purified tuberculin RT 19-21 is composed of at least two fractions of different adsorbability.It is pointed out that the accuracy of tuberculin testing would be markedly increased if adsorption from the tuberculin dilutions could be prevented.
已发现纯化结核菌素RT 19 - 21（丹麦国家血清研究所，哥本哈根）的稀释液在效价上存在非系统性变化。初步调查表明，结核菌素吸附到容器上可能是原因所在。本文报道了在豚鼠身上进行的一系列实验，对这种可能性进行了进一步探究。事实证明，结核菌素确实会吸附到容器的玻璃表面：用含有吐温80的缓冲洗脱液从先前用于结核菌素的安瓿中获得了具有结核菌素活性的洗脱液。估计表明，RT 19 - 21的5 TU稀释液（每0.1 ml制备成含5 TU或0.0001 mg）平均因吸附而损失60%的活性物质。不同安瓿的吸附量不同，并且观察到安瓿中原始内容物的效价与用吐温80洗脱液重新填充安瓿时获得的洗脱液效价之间呈负相关。吸附程度取决于稀释液的强度、温度以及稀释液与容器接触的持续时间和程度。另一个观察结果是，摇晃安瓿会减少吸附。有迹象表明，纯化结核菌素RT 19 - 21至少由两种吸附性不同的组分组成。有人指出，如果能够防止结核菌素稀释液的吸附，结核菌素检测的准确性将显著提高。
