Guevara P, Alonso G, da Silveira J F, de Mello M, Scorza J V, Añez N, Ramírez J L
Centro de Biología Celular, Universidad Central de Venezuela, Caracas.
Mol Biochem Parasitol. 1992 Nov;56(1):15-26. doi: 10.1016/0166-6851(92)90150-i.
DNA probes from the nontranscribed ribosomal spacer (NTS), of Leishmania garnhami and Leishmania braziliensis were constructed and tested for sensitivity and specificity against different Leishmania isolates. The L. garnhami probes were species-specific under hybridization conditions of high stringency, but displayed specificity for the mexicana complex under conditions of intermediate stringency. The L. braziliensis probes showed 'complex' specificity. RFLP for the nontranscribed spacer within the braziliensis complex revealed very homogeneous patterns even for organisms currently accepted as different species. A PCR assay for the detection of Leishmania from the braziliensis complex is presented.
构建了来自杜氏利什曼原虫和巴西利什曼原虫非转录核糖体间隔区（NTS）的DNA探针，并针对不同的利什曼原虫分离株测试了其敏感性和特异性。杜氏利什曼原虫探针在高严格度杂交条件下具有种特异性，但在中等严格度条件下对墨西哥利什曼原虫复合体显示出特异性。巴西利什曼原虫探针表现出“复合体”特异性。巴西利什曼原虫复合体内非转录间隔区的RFLP分析显示，即使对于目前被认为是不同物种的生物体，其模式也非常一致。本文介绍了一种用于检测巴西利什曼原虫复合体中利什曼原虫的PCR检测方法。
