Wang J Y, Chen W Y
Department of Pediatrics, National Cheng-Kung University Medical Center, Tainan, Taiwan, R.O.C.
J Formos Med Assoc. 1992 Dec;91(12):1127-32.
The multiple allergosorbent chemiluminescent assay (MAST-CLA) is a method for measuring total and allergen-specific IgE in human serum by a chemiluminescent immuno-enzymatic system. To compare the results of MAST tests with those of radioallergosorbent (RAST) and skin tests as an adjunct to the diagnosis of inhalant allergens in Taiwan, 195 asthmatic children, aged 5 to 15 years, were studied. All MAST tests had valid positive and negative control threads. The most important allergens in our patients were the two mite species: Dermatophagoid pteronyssinus and Dermatophagoid farinae (87.5% and 82.1%, respectively). There were also large responses (MAST-CLA class > or = 2) to the cockroach mix (28.45%), Alternaria (24.3%), and Eucalyptus (32.6%). The individual efficiency percentage between MAST-CLA and skin tests was D. pteronyssinus 91%, D. farinae 88%, cockroach mix 85%, feather mix 75%, dog dander 64%, Candida 75%, Aspergillus 79%, Alternaria 88%, ragweed mix 62% and Eucalyptus 76%. Comparison of the results of allergen-specific IgE measured by MAST-CLA and RAST were also significantly correlated for all four allergens (D. farinae, Candida, Alternaria and grass mix, r = 0.79-0.92). MAST-CLA was randomly duplicated and proven reproducible in 89% of the tests. Changes between positive and negative results occurred in only 3.6% of the tests. MAST-CLA is a simple in vitro test for specific IgE to 35 allergens, which compares favorably with RAST. It is concluded that MAST-CLA and RAST are similar in their ability to measure allergen-specific IgE, and correlate equally well with skin tests and clinical history in asthmatic children.
多过敏原吸附化学发光测定法（MAST-CLA）是一种通过化学发光免疫酶系统测定人血清中总IgE和过敏原特异性IgE的方法。为了比较MAST检测结果与放射变应原吸附试验（RAST）及皮肤试验结果，以辅助诊断台湾地区吸入性过敏原，对195名5至15岁的哮喘儿童进行了研究。所有MAST检测均有有效的阳性和阴性对照线。我们患者中最重要的过敏原是两种螨虫：屋尘螨和粉尘螨（分别为87.5%和82.1%）。对蟑螂混合物（28.45%）、链格孢属（24.3%）和桉属（32.6%）也有较大反应（MAST-CLA分级≥2）。MAST-CLA与皮肤试验之间的个体有效率百分比分别为：屋尘螨91%、粉尘螨88%、蟑螂混合物85%、羽毛混合物75%、狗皮屑64%、念珠菌75%、曲霉属79%、链格孢属88%、豚草混合物62%和桉属76%。MAST-CLA和RAST检测的过敏原特异性IgE结果比较，对所有四种过敏原（粉尘螨、念珠菌、链格孢属和草混合物，r = 0.79 - 0.92）也有显著相关性。MAST-CLA随机重复检测，89% 的检测结果可重复。阳性和阴性结果之间的变化仅发生在3.6% 的检测中。MAST-CLA是一种针对35种过敏原特异性IgE的简单体外检测方法，与RAST相比具有优势。结论是，MAST-CLA和RAST在测量过敏原特异性IgE的能力方面相似，并且在哮喘儿童中与皮肤试验和临床病史的相关性同样良好。
