Agata H, Yomo A, Hanashiro Y, Muraki T, Kondo N, Orii T
Department of Pediatrics, Okinawa Prefectural Nanbu Hospital, Itoman, Japan.
Ann Allergy. 1993 Feb;70(2):153-7.
The MAST chemiluminescent assay (CLA) system uses enzyme-linked antihuman IgE and a chemiluminogenic substance to determine specific IgE antibodies. The MAST CLA system therefore not only makes unnecessary the precautions required for handling isotopic materials but has the advantage of long-term reagent stability. The MAST CLA system assay protocol consists of three steps: overnight incubation of serum, a 4-hour incubation with enzyme-labeled antibody, and a 30-minute chemiluminescent reaction, which produces a visible image (immunograph) on high-speed instant Polaroid film. We evaluated this MAST CLA system in 47 allergic children and compared the results with those for RAST and skin tests. Good correlations were found between MAST and RAST for house dust, Dermatophagoides farinae, cat, Japanese cedar pollen, egg white, and soybeans, the correlation coefficients being greater than 0.60. When the diagnostic results for MAST and RAST were compared with those of skin test reactions for house dust, Dermatophagoides farinae, cat, Japanese cedar pollen, egg white, and soybeans, MAST had a sensitivity of 85% and a specificity of 82% as compared with 88% and 83% for RAST. Further, an average of 2.1 different allergens were detected in one serum sample. These results suggest that the MAST CLA system can be used to detect specific IgE antibodies and that it provides accurate results for allergic children.
MAST化学发光免疫分析（CLA）系统采用酶联抗人IgE和化学发光物质来测定特异性IgE抗体。因此，MAST CLA系统不仅无需采取处理同位素材料所需的预防措施，而且具有试剂长期稳定的优点。MAST CLA系统的检测方案包括三个步骤：血清过夜孵育、与酶标记抗体孵育4小时以及30分钟的化学发光反应，该反应会在高速即时宝丽来胶片上产生可见图像（免疫图谱）。我们对47名过敏儿童进行了MAST CLA系统评估，并将结果与放射变应原吸附试验（RAST）和皮肤试验的结果进行了比较。发现MAST与RAST在对屋尘、粉尘螨、猫、日本柳杉花粉、蛋清和大豆的检测上具有良好的相关性，相关系数大于0.60。当将MAST和RAST的诊断结果与屋尘、粉尘螨、猫、日本柳杉花粉、蛋清和大豆的皮肤试验反应结果进行比较时，MAST的灵敏度为85%，特异性为82%，而RAST的灵敏度和特异性分别为88%和83%。此外，一份血清样本平均能检测出2.1种不同的过敏原。这些结果表明，MAST CLA系统可用于检测特异性IgE抗体，并且能为过敏儿童提供准确的结果。
