KAPRAL F A, SHAYEGANI M G
J Exp Med. 1959 Jul 1;110(1):123-38. doi: 10.1084/jem.110.1.123.
A tissue culture procedure is described which permits the quantitative evaluation of the intracellular survival of staphylococci within leucocytes. Staphylococcus aureus survived, but did not multiply, within neutrophils and monocytes of normal rabbits. The same was true of normal human blood leucocytes. Staphylococcus albus on the other hand was destroyed by these cells under the same conditions. Rat monocytes destroyed S. aureus and S. albus with equal facility. Although most experiments were carried out in the presence of 50 microg. streptomycin/ml., similar results were obtained without the use of this antibiotic. The applications of the tissue culture procedure with regard to studies on virulence and immunity in staphylococcal disease are discussed.
本文描述了一种组织培养方法，该方法可对葡萄球菌在白细胞内的细胞内存活情况进行定量评估。金黄色葡萄球菌可在正常兔的中性粒细胞和单核细胞内存活，但不会增殖。正常人血白细胞也有同样情况。另一方面，在相同条件下，白色葡萄球菌会被这些细胞破坏。大鼠单核细胞对金黄色葡萄球菌和白色葡萄球菌的破坏能力相同。尽管大多数实验是在每毫升含50微克链霉素的情况下进行的，但在不使用这种抗生素时也获得了类似结果。本文还讨论了该组织培养方法在葡萄球菌病毒力和免疫研究中的应用。
