Goochee C F, Monica T
Department of Chemical Engineering, Stanford University, CA 94305-5025.
Biotechnology (N Y). 1990 May;8(5):421-7. doi: 10.1038/nbt0590-421.
Cultured mammalian cells are being used to produce proteins for therapeutic and diagnostic use because of their ability to perform complex post-translational modifications, including glycosylation. The oligosaccharide moieties can play an important role in defining several biological properties of glycoproteins, including clearance rate, immunogenicity, and biological specific activity. There is a growing interest in defining the factors that influence glycosylation, including the cell culture environment. In this review we organize the published data from in vitro cell culture and tissue culture studies that demonstrate direct effects of the culture environment on N-linked glycosylation.
培养的哺乳动物细胞正被用于生产用于治疗和诊断的蛋白质，因为它们能够进行复杂的翻译后修饰，包括糖基化。寡糖部分在定义糖蛋白的几种生物学特性方面可以发挥重要作用，包括清除率、免疫原性和生物学比活性。人们越来越关注确定影响糖基化的因素，包括细胞培养环境。在这篇综述中，我们整理了来自体外细胞培养和组织培养研究的已发表数据，这些数据证明了培养环境对N-连接糖基化的直接影响。
