Osborne J C, Rupprecht C E, Olson J G, Ksiazek T G, Rollin P E, Niezgoda M, Goldsmith C S, An U S, Nichol S T
Special Pathogens Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, Georgia 30333, USA.
Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, Georgia 30333, USA.
J Gen Virol. 2003 Oct;84(Pt 10):2685-2689. doi: 10.1099/vir.0.19294-0.
A virus isolated from dead Chaerephon plicata bats collected near Kampot, Cambodia, was identified as a member of the family Bunyaviridae by electron microscopy. The only bunyavirus previously isolated from Chaerephon species bats in South-East Asia is Kaeng Khoi (KK) virus (genus Orthobunyavirus), detected in Thailand over 30 years earlier and implicated as a public health problem. Using RT-PCR, nucleotide sequences from the M RNA segment of several virus isolates from the Cambodian C. plicata bats were found to be almost identical and to differ from those of the prototype KK virus by only 2.6-3.2 %, despite the temporal and geographic separation of the viruses. These results identify the Cambodian bat viruses as KK virus, extend the known virus geographic range and document the first KK virus isolation in 30 years. These genetic data, together with earlier serologic data, show that KK viruses represent a distinct group within the genus Orthobunyavirus.
从柬埔寨贡布附近采集的死亡皱唇犬吻蝠身上分离出的一种病毒，经电子显微镜鉴定为布尼亚病毒科的一员。此前在东南亚从犬吻蝠属蝙蝠中分离出的唯一布尼亚病毒是孔敬（KK）病毒（正布尼亚病毒属），30多年前在泰国被检测到，并被认为是一个公共卫生问题。使用逆转录聚合酶链反应（RT-PCR）发现，来自柬埔寨皱唇犬吻蝠的几种病毒分离株的M RNA片段的核苷酸序列几乎相同，与原型KK病毒的序列仅相差2.6%-3.2%，尽管这些病毒在时间和地理上存在分隔。这些结果将柬埔寨蝙蝠病毒鉴定为KK病毒，扩展了已知病毒的地理范围，并记录了30年来首次分离出KK病毒。这些遗传数据与早期的血清学数据一起表明，KK病毒代表正布尼亚病毒属内的一个独特群体。
