Degelau A, Freitag R, Linz F, Middendorf C, Scheper T, Bley T, Müller S, Stoll P, Reardon K F
Institut für Technische Chemie, Universität Hannover, Germany.
J Biotechnol. 1992 Aug;25(1-2):115-44. doi: 10.1016/0168-1656(92)90112-m.
In this article, the applications of immunoanalysis and flow cytometry for research and process monitoring in biotechnology are discussed. Brief reviews of the two analytical methods are followed by descriptions of actual applications in various areas of biotechnology. In the case of immunoanalysis, emphasis is placed on systems for on-line bioprocess monitoring, and examples are given for a thermostable pullulanase, a mouse IgG, and antithrombin III. Although flow cytometry is not currently an on-line analytical technique, its value as an off-line method is illustrated by examples of the measurement of shear stress effects, lipid content, and sterol content.
本文讨论了免疫分析和流式细胞术在生物技术研究和过程监测中的应用。在对这两种分析方法进行简要综述之后，描述了它们在生物技术各个领域的实际应用。对于免疫分析，重点介绍了在线生物过程监测系统，并给出了嗜热支链淀粉酶、小鼠IgG和抗凝血酶III的示例。虽然流式细胞术目前不是一种在线分析技术，但通过测量剪切应力效应、脂质含量和甾醇含量的示例说明了其作为离线方法的价值。
