Schweder T, Schmidt I, Herrmann H, Neubauer P, Hecker M, Hofmann K
Institut für Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, Federal Republic of Germany.
Appl Microbiol Biotechnol. 1992 Oct;38(1):91-3. doi: 10.1007/BF00169425.
A cloning vector system was constructed on the basis of the pBR322 derivative pEG1 by introducing the whole parB locus of plasmid R1 cloned behind the promoter of the alkaline phosphatase gene (phoA) of Escherichia coli. The parB locus in combination with the phoA promoter ensures both (i) plasmid stabilization due to the post-segregational killing of plasmid-free cells during growth and (ii) killing of the cells induced by the potential environmental signal phosphate limitation. This vector, therefore, appears to be a model system for increasing the stability of recombinant plasmids and for decreasing the potential risks in the application of recombinant bacteria in industrial fermentations.
基于pBR322衍生物pEG1构建了一种克隆载体系统，方法是将克隆的质粒R1的整个parB基因座引入大肠杆菌碱性磷酸酶基因（phoA）启动子的下游。parB基因座与phoA启动子相结合，确保了：（i）由于生长过程中无质粒细胞的后分离杀伤而实现质粒稳定，以及（ii）由潜在环境信号磷酸盐限制诱导的细胞杀伤。因此，该载体似乎是一种用于提高重组质粒稳定性以及降低重组细菌在工业发酵应用中潜在风险的模型系统。
