Manso M A, San Román J I, de Dios I, García L J, López M A
Departamento de Fisiología y Farmacología, Facultad de Biología, Universidad de Salamanca, Spain.
Dig Dis Sci. 1992 Mar;37(3):364-8. doi: 10.1007/BF01307729.
A study was made with different doses of cerulein (2, 4, 10 and 20 micrograms/kg) administered subcutaneously to rats by four injections at intervals of 1 hr; the aim of this work was to study exocrine pancreatic secretion of the rat under cerulein-induced acute pancreatitis, analyzing enzyme and hydroelectrolyte secretion of pancreatic juice. A further aim was to study the relationship between the dose of cerulein and the plasma levels of peptides controlling hydroelectrolyte secretion of the pancreas, like secretin and vasoactive intestinal peptide (VIP). At the lowest dose schedule, the amounts of total protein and enzymes (amylase and trypsin) in pancreatic juice decreased significantly, plasma amylase increased, and the pancreas became edematous. Higher doses magnified these effects. By contrast, ductular function (flow and HCO3-) was well preserved in cerulein-treated rats, and this was probably due to the significant increase in plasma levels of immunoreactive secretin whereas VIP levels were unchanged. The secretin released by treatment with cerulein is able to palliate the lack of flow from acinar origin that is affected in the process of acute pancreatitis, being a beneficial response to the cerulein treatment.
对大鼠皮下注射不同剂量的雨蛙素（2、4、10和20微克/千克），每隔1小时注射一次，共注射四次；这项研究的目的是研究雨蛙素诱导的急性胰腺炎大鼠的胰腺外分泌，分析胰液的酶和水电解质分泌。另一个目的是研究雨蛙素剂量与控制胰腺水电解质分泌的肽（如促胰液素和血管活性肠肽（VIP））的血浆水平之间的关系。在最低剂量方案下，胰液中总蛋白和酶（淀粉酶和胰蛋白酶）的量显著减少，血浆淀粉酶增加，胰腺出现水肿。更高的剂量放大了这些效应。相比之下，雨蛙素处理的大鼠的导管功能（流量和HCO3-）得到了很好的保留，这可能是由于免疫反应性促胰液素的血浆水平显著增加，而VIP水平未改变。雨蛙素处理释放的促胰液素能够缓解急性胰腺炎过程中受影响的腺泡来源的流量不足，这是对雨蛙素处理的有益反应。
