Nelson R E, Lehman J F, Metzenberg R L
Genetics. 1976 Oct;84(2):183-92. doi: 10.1093/genetics/84.2.183.
A mutant of Neurospora crassa with an altered repressible acid phosphatase has been isolated. The enzyme is much more thermolabile than that of wild type, and has an increased Michaelis constant. Tests of allelic interactions (in partial diploids) and in vitro mixing experiments were consistent with the mutation being in the structural gene for the enzyme. This gene, pho-3, was found to be located in the right arm of Linkage Group IV (LGIV). Thus, pho-3 and the structural gene for repressible alkaline phosphatase, pho-2 (LG V), map in separate linkage groups and cannot be part of the same operon. Neither of these structural genes is linked to the known regulatory genes, nuc-1 (LG I), nuc-2 (LG II), and preg (LG II).
已分离出一种粗糙脉孢菌的突变体，其可阻遏酸性磷酸酶发生了改变。该酶比野生型的酶对热更不稳定，且米氏常数增加。等位基因相互作用测试（在部分二倍体中）和体外混合实验表明，该突变位于该酶的结构基因中。发现这个基因pho - 3位于连锁群IV（LGIV）的右臂。因此，pho - 3和可阻遏碱性磷酸酶的结构基因pho - 2（LG V）位于不同的连锁群中，不可能是同一操纵子的一部分。这两个结构基因均与已知的调控基因nuc - 1（LG I）、nuc - 2（LG II）和preg（LG II）不连锁。
