van Setten G B, Koch J W, Tervo K, Lang G K, Tervo T, Naumann G O, Kolkmeier J, Virtanen I, Tarkkanen A
Department of Ophthalmology, University of Helsinki, Finland.
Graefes Arch Clin Exp Ophthalmol. 1992;230(2):178-83. doi: 10.1007/BF00164660.
In order to investigate the effects of excimer laser surgery on corneal wound healing, 25 rabbits underwent anterior keratectomy at a depth of 100 or 300 microns with a Meditec MEL 50 excimer laser. After various intervals the animals were killed and the cornea excised and investigated immunohistochemically for the expression of extracellular matrix (ECM) proteins, fibronectin and tenascin. Fibronectin was shown to occur earlier than tenascin, and the two also had different distribution patterns. Wound depth showed no clear effect on the localization and time of ECM protein expression. This study indicates that corneal wounds caused by excimer laser radiation and those caused by mechanical surgery differ as to healing mechanisms.
为了研究准分子激光手术对角膜伤口愈合的影响，25只兔子用Meditec MEL 50准分子激光进行了深度为100或300微米的前板层角膜切除术。在不同时间段后处死动物，切除角膜并进行免疫组织化学检查，以检测细胞外基质（ECM）蛋白、纤连蛋白和腱生蛋白的表达。结果显示纤连蛋白比腱生蛋白出现得更早，并且二者的分布模式也不同。伤口深度对ECM蛋白表达的定位和时间没有明显影响。这项研究表明，准分子激光辐射造成的角膜伤口与机械手术造成的角膜伤口在愈合机制方面存在差异。
