MELLY M A, THOMISON J B, ROGERS D E
J Exp Med. 1960 Dec 1;112(6):1121-30. doi: 10.1084/jem.112.6.1121.
Direct observations by phase microscopy have demonstrated that small numbers of pathogenic staphylococci survive prolonged periods of time within living human polymorphonuclear leukocytes. Non-pathogenic microorganisms are rapidly destroyed in similar preparations. Leukocytes in which staphylococci remained viable often appeared less vigorous after ingesting microorganisms, but intracellular survival could not be correlated with obvious leukocyte damage with any consistency. Both pathogenic and non-pathogenic cocci were seen to divide within living granulocytes during the first few minutes after ingestion. Occasionally pathogenic staphylococci multiplied in dying cells after long periods of intracellular residence. Phagocytosis of more than one pair of staphylococci by a single leukocyte appeared to act as a stimulus to bacterial destruction. Multiple ingestions of pathogenic staphylococci reduced the incidence of survival of the total microbiol population contained within the cell.
相差显微镜的直接观察表明，少量致病性葡萄球菌可在人类活的多形核白细胞内存活很长时间。在类似的标本中，非致病性微生物会迅速被破坏。葡萄球菌仍能存活的白细胞在摄取微生物后通常显得活力较差，但细胞内存活与明显的白细胞损伤之间并无一致的相关性。在摄取后的最初几分钟内，致病性和非致病性球菌都可见在活的粒细胞内分裂。偶尔，致病性葡萄球菌在细胞内长期留存后会在濒死细胞中繁殖。单个白细胞吞噬多对葡萄球菌似乎可刺激细菌被破坏。多次摄取致病性葡萄球菌可降低细胞内所含全部微生物群体的存活发生率。
