Brezinschek R I, Lipsky P E, Galea P, Vita R, Oppenheimer-Marks N
Harold C. Simmons Arthritis Research Center, University of Texas Southwestern Medical Center, Dallas 75235.
J Immunol. 1995 Apr 1;154(7):3062-77.
The phenotype of CD4+ T cells capable of transendothelial migration was determined using an in vitro model system, in which cells migrate through a monolayer of endothelial cells (EC) on collagen gels. A specific subset of resting CD4+ memory T cells was found to migrate. T cells within this subset can be defined by the bright expression of CD11a, CD26, CD44, and CD49d. Additionally, the migratory CD4+ T cell population is largely CD58bright, CD31-, CD62L-, and is also enriched in cells that brightly express CD49c, CD49e, and CD49f. Only a minority of the cells are activated, as indicated by expression of CD69. The EC were found to play a central role in facilitating migration of this subset because selective enrichment of CD11abright, CD26bright, CD44bright, CD4+ T cells was not observed when cells migrated in the absence of EC. Activation of the T cells induced a modest degree of migration of an additional subset of CD45RA+, CD31+ naive T cells. In contrast, TNF-alpha activation of the EC increased the transendothelial migration of an additional subset of activated memory T cells that expressed CD69 and CD62L. Neither activation of the T cells, stimulation of the EC, nor the presence of macrophage inflammatory protein-1 alpha (MIP-1 alpha) or RANTES, however, altered the phenotype of the majority of the migratory CD4+ T cell population, which is characteristic of a particular stage of memory cell differentiation. These results suggest that CD4+ T cells acquire the capacity for transendothelial migration at a specific phase of maturation that is only minimally altered by the activation of either the T cell or the EC, or by the presence of specific chemokines in the subendothelial matrix.
利用体外模型系统确定了能够进行跨内皮迁移的CD4+T细胞的表型，在该模型系统中，细胞通过胶原凝胶上的单层内皮细胞（EC）迁移。发现静息CD4+记忆T细胞的一个特定亚群能够迁移。该亚群内的T细胞可通过CD11a、CD26、CD44和CD49d的明亮表达来定义。此外，迁移的CD4+T细胞群体在很大程度上是CD58bright、CD31-、CD62L-，并且还富含明亮表达CD49c、CD49e和CD49f的细胞。如CD69的表达所示，只有少数细胞被激活。发现EC在促进该亚群的迁移中起核心作用，因为当细胞在没有EC的情况下迁移时，未观察到CD11abright、CD26bright、CD44bright、CD4+T细胞的选择性富集。T细胞的激活诱导了另外一个CD45RA+、CD31+幼稚T细胞亚群的适度迁移。相比之下，EC的TNF-α激活增加了另外一个表达CD69和CD62L的活化记忆T细胞亚群的跨内皮迁移。然而，无论是T细胞的激活、EC的刺激，还是巨噬细胞炎性蛋白-1α（MIP-1α）或RANTES的存在，都没有改变大多数迁移的CD4+T细胞群体的表型，这是记忆细胞分化特定阶段的特征。这些结果表明，CD4+T细胞在成熟过程的特定阶段获得跨内皮迁移的能力，该阶段仅因T细胞或EC的激活或内皮下基质中特定趋化因子的存在而发生最小程度的改变。
