van Dam A P, Kuiper H, Vos K, Widjojokusumo A, de Jongh B M, Spanjaard L, Ramselaar A C, Kramer M D, Dankert J
Department of Medical Microbiology, University of Amsterdam, The Netherlands.
Clin Infect Dis. 1993 Oct;17(4):708-17. doi: 10.1093/clinids/17.4.708.
Borrelia burgdorferi sensu lato has been subdivided into three genospecies: B. burgdorferi sensu stricto, B. garinii, and B. burgdorferi group VS461. Sixty-eight isolates cultured from patients and 26 strains from ticks were characterized with use of SDS-PAGE, western blotting, and rRNA gene restriction analysis. Fifty-seven of 58 strains obtained from the skin of 70 patients who had erythema migrams or acrodermatitis chronica atrophicans were of group VS461, whereas the genotype of the remaining strain was unidentifiable. Of 10 strains cultured from CSF (n = 3) and skin (n = 7) of 20 patients with extracutaneous symptoms of Lyme borreliosis, nine were B. garinii and one was B. burgdorferi sensu stricto. Of these 20 patients, 17 had neuroborreliosis, one had arthritis and carditis, one had myalgia, and one had erythema and arthralgia. All 26 isolates from ticks were of group VS461. In conclusion, infections due to group VS461 and B. garinii are associated with cutaneous and extracutaneous symptoms, respectively. Our findings suggest that B. burgdorferi genotypes have different pathogenic potentials.
狭义伯氏疏螺旋体、伽氏疏螺旋体和伯氏疏螺旋体VS461群。使用SDS - PAGE、蛋白质免疫印迹和rRNA基因限制性分析对从患者分离培养的68株菌株以及从蜱虫分离的26株菌株进行了鉴定。从70例患有游走性红斑或慢性萎缩性肢皮炎的患者皮肤中获得的58株菌株中有57株属于VS461群，而其余菌株的基因型无法鉴定。在从20例有莱姆病皮肤外症状的患者的脑脊液（n = 3）和皮肤（n = 7）中培养的10株菌株中，9株是伽氏疏螺旋体，1株是狭义伯氏疏螺旋体。在这20例患者中，17例有神经型莱姆病，1例有关节炎和心肌炎，1例有肌痛，1例有红斑和关节痛。所有从蜱虫分离的26株菌株均属于VS461群。总之，VS461群和伽氏疏螺旋体引起的感染分别与皮肤症状和皮肤外症状相关。我们的研究结果表明，伯氏疏螺旋体基因型具有不同的致病潜力。
