GAYLORD W H, HSIUNG G D
J Exp Med. 1961 Dec 1;114(6):987-96. doi: 10.1084/jem.114.6.987.
Cells infected with the vacuolating virus, SV(40), respond by swelling to several times their normal volume. Within enlarged nuclei, virus-containing inclusions appear which are acidophilic and Feulgen-positive. The formation of nuclear inclusions is followed by the appearance of cytoplasmic vacuoles and then shrinkage of the cell. Inclusions were found to exhibit unique double staining when a light-green counterstain was used in the Feulgen reaction. The virus is of low electron density, round, and 300 A in diameter. It occurs in large numbers, singly and in short chains, and it appears to multiply at the expense of chromatin.
感染空泡病毒SV(40)的细胞会肿胀至正常体积的数倍。在增大的细胞核内，出现含病毒的包涵体，这些包涵体呈嗜酸性且对福尔根反应呈阳性。核内包涵体形成后，接着出现细胞质空泡，然后细胞萎缩。当在福尔根反应中使用淡绿色复染剂时，发现包涵体呈现独特的双重染色。该病毒电子密度低，呈圆形，直径为300埃。它大量存在，单个或呈短链状，似乎以染色质为代价进行增殖。
