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基于单克隆抗体的捕获ELISA/ELIFA法检测临床标本中的伯氏考克斯氏体。

Monoclonal antibody based capture ELISA/ELIFA for detection of Coxiella burnetii in clinical specimens.

作者信息

Thiele D, Karo M, Krauss H

机构信息

Institut für Hygiene und Infektionskrankheiten der Tiere, Justus Liebig Universität, Giessen, F.R.G.

出版信息

Eur J Epidemiol. 1992 Jul;8(4):568-74. doi: 10.1007/BF00146378.

DOI:10.1007/BF00146378
PMID:1397227
Abstract

A CAPTURE ELISA/ELIFA system based on monoclonal capture and biotinylated monoclonal detection antibody is described. The assay is fast, highly specific and detects a minimum dose of 2500 Coxiella (C.) burnetii particles. In contrast to the sophisticated and cumbersome isolation procedures, even non-specialized laboratories could use this assay system for investigating clinical samples of different origin for C. burnetii within a short period of time.

摘要

本文描述了一种基于单克隆捕获抗体和生物素化单克隆检测抗体的捕获酶联免疫吸附测定/酶联免疫荧光测定（ELISA/ELIFA）系统。该检测方法快速、特异性高，能检测到最低2500个伯氏考克斯体（C. burnetii）颗粒的剂量。与复杂繁琐的分离程序不同，即使是非专业实验室也可在短时间内使用该检测系统对不同来源的临床样本进行伯氏考克斯体检测。

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本文引用的文献

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[Continuous multiplication of Coxiella burnetii through persisting infection in buffalo-green monkey (BGM) cell cultures].[通过在水牛绿猴（BGM）细胞培养物中的持续感染实现伯氏考克斯氏体的连续增殖]
Zentralbl Veterinarmed B. 1983 Mar;30(2):109-16.
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Detection and persistence of specific IgM antibody to Coxiella burnetii by enzyme-linked immunosorbent assay: a comparison with immunofluorescence and complement fixation tests.通过酶联免疫吸附测定法检测伯纳特立克次体特异性IgM抗体及其持续性：与免疫荧光法和补体结合试验的比较
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Q fever and Coxiella burnetii: a model for host-parasite interactions.
抗菌肽作为筛选难培养物种的新型识别分子。
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Nonculture Laboratory Methods for the Diagnosis of Infectious Endocarditis.用于诊断感染性心内膜炎的非培养实验室方法。
Curr Infect Dis Rep. 1999 Jun;1(2):136-141. doi: 10.1007/s11908-996-0020-x.
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Q fever.Q热
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PCR detection of Coxiella burnetii from different clinical specimens, especially bovine milk, on the basis of DNA preparation with a silica matrix.基于硅胶基质DNA制备，对不同临床标本（尤其是牛乳）中的伯氏考克斯体进行PCR检测。
Appl Environ Microbiol. 1998 Nov;64(11):4234-7. doi: 10.1128/AEM.64.11.4234-4237.1998.
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Diagnosis of Q fever.Q热的诊断。
J Clin Microbiol. 1998 Jul;36(7):1823-34. doi: 10.1128/JCM.36.7.1823-1834.1998.
8
Monoclonal antibodies to Coxiella burnetii that cross-react with strain Nine Mile.与九英里菌株发生交叉反应的针对伯纳特立克次体的单克隆抗体。
Clin Diagn Lab Immunol. 1995 Sep;2(5):531-4. doi: 10.1128/cdli.2.5.531-534.1995.
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Plasmid based differentiation and detection of Coxiella burnetii in clinical samples.基于质粒的临床样本中伯氏考克斯体的鉴别与检测
Eur J Epidemiol. 1993 Jul;9(4):411-8. doi: 10.1007/BF00157399.
Q热与贝纳柯克斯体：宿主-寄生虫相互作用的一个模型
Microbiol Rev. 1983 Jun;47(2):127-49. doi: 10.1128/mr.47.2.127-149.1983.
4
Enzyme immunoassay for surveillance of Q fever.用于Q热监测的酶免疫测定法。
Am J Vet Res. 1985 Nov;46(11):2413-7.
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Poisson statistical analysis of repetitive subcloning by the limiting dilution technique as a way of assessing hybridoma monoclonality.采用有限稀释技术对重复亚克隆进行泊松统计分析，以此作为评估杂交瘤单克隆性的一种方法。
Methods Enzymol. 1986;121:412-7. doi: 10.1016/0076-6879(86)21039-3.
6
In vitro susceptibility of Coxiella burnetii to antibiotics, including several quinolones.伯纳特柯克斯体对包括几种喹诺酮类药物在内的抗生素的体外敏感性。
Antimicrob Agents Chemother. 1987 Jul;31(7):1079-84. doi: 10.1128/AAC.31.7.1079.
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Animal models in Q fever: pathological responses of inbred mice to phase I Coxiella burnetii.Q热的动物模型：近交系小鼠对I相伯纳特立克次体的病理反应
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Comparative virulence of intra- and interstrain lipopolysaccharide variants of Coxiella burnetii in the guinea pig model.伯纳特立克次氏体菌株内和菌株间脂多糖变体在豚鼠模型中的比较毒力
Infect Immun. 1987 May;55(5):1144-50. doi: 10.1128/iai.55.5.1144-1150.1987.
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Humoral immune response to Q fever: enzyme-linked immunosorbent assay antibody response to Coxiella burnetii in experimentally infected guinea pigs.对Q热的体液免疫反应：实验感染豚鼠中针对伯氏考克斯体的酶联免疫吸附测定抗体反应
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Diagn Microbiol Infect Dis. 1986 Feb;4(2):125-32. doi: 10.1016/0732-8893(86)90146-x.