基于单克隆抗体的捕获ELISA/ELIFA法检测临床标本中的伯氏考克斯氏体。
Monoclonal antibody based capture ELISA/ELIFA for detection of Coxiella burnetii in clinical specimens.
作者信息
Thiele D, Karo M, Krauss H
机构信息
Institut für Hygiene und Infektionskrankheiten der Tiere, Justus Liebig Universität, Giessen, F.R.G.
出版信息
Eur J Epidemiol. 1992 Jul;8(4):568-74. doi: 10.1007/BF00146378.
A CAPTURE ELISA/ELIFA system based on monoclonal capture and biotinylated monoclonal detection antibody is described. The assay is fast, highly specific and detects a minimum dose of 2500 Coxiella (C.) burnetii particles. In contrast to the sophisticated and cumbersome isolation procedures, even non-specialized laboratories could use this assay system for investigating clinical samples of different origin for C. burnetii within a short period of time.
本文描述了一种基于单克隆捕获抗体和生物素化单克隆检测抗体的捕获酶联免疫吸附测定/酶联免疫荧光测定（ELISA/ELIFA）系统。该检测方法快速、特异性高，能检测到最低2500个伯氏考克斯体（C. burnetii）颗粒的剂量。与复杂繁琐的分离程序不同，即使是非专业实验室也可在短时间内使用该检测系统对不同来源的临床样本进行伯氏考克斯体检测。
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