Hirst C F, Poller L
United Kingdom Reference Laboratory for Anticoagulant Reagents and Control, Withington Hospital, Manchester.
J Clin Pathol. 1992 Aug;45(8):701-3. doi: 10.1136/jcp.45.8.701.
To investigate the cause of turbidity in reconstituted lyophilised plasmas and to determine its effect on coagulometers.
The turbidities of 20 normal plasmas and 16 reconstituted lyophilised plasmas were determined by comparing a 1 in 4 dilution in distilled water with a standard suspension in an Aminco Fluorocolorimeter (American Instrument Co) in nephelometric mode. The turbidities of five other plasmas were determined before and after lyophilisation. The turbid components of fresh and reconstituted lyophilised plasmas were studied using electron microscopy. The effects of turbidity on five types of coagulometer were determined by adding varying concentrations of a turbidity enhancing material.
Reconstituted lyophilised plasmas were more turbid than normal plasmas, because of agglomerated liposomes. Serum depleted of chylomicrons and very low density lipoproteins was not rendered more turbid by lyophilisation. Three out of five types of automated coagulometer tested gave activated partial thromboplastin times which were appreciably affected by plasma turbidity. One of the instruments was unable to detect a clot in a moderately turbid plasma. A second instrument gave results which were significantly affected by turbidity. Turbidity of the substrate plasma did not affect specific factor VIII assays in two types of coagulometer.
Lyophilisation of plasma induces turbidity due to the agglomeration of lipids. Such turbidity can affect the results of coagulation tests. Suppliers of lyophilised plasmas should be aware of this problem.
研究复溶冻干血浆浑浊的原因，并确定其对凝血分析仪的影响。
通过在氨基柯荧光比色计（美国仪器公司）中采用比浊法模式，将20份正常血浆和16份复溶冻干血浆在蒸馏水中的1:4稀释液与标准悬浮液进行比较，测定其浊度。对另外五份血浆在冻干前后测定其浊度。使用电子显微镜研究新鲜和复溶冻干血浆的浑浊成分。通过添加不同浓度的浑浊增强物质，测定浑浊对五种凝血分析仪的影响。
由于脂质体聚集，复溶冻干血浆比正常血浆更浑浊。去除乳糜微粒和极低密度脂蛋白的血清冻干后并未变得更浑浊。在测试的五种自动凝血分析仪中，有三种给出的活化部分凝血活酶时间明显受血浆浑浊度影响。其中一台仪器无法检测到中度浑浊血浆中的凝块。另一台仪器给出的结果受浑浊度影响显著。底物血浆的浑浊度在两种凝血分析仪中不影响特定因子VIII测定。
血浆冻干会因脂质聚集而导致浑浊。这种浑浊会影响凝血试验结果。冻干血浆供应商应意识到这个问题。
