Weglenski P, Tyler B
J Bacteriol. 1977 Feb;129(2):880-7. doi: 10.1128/jb.129.2.880-887.1977.
We examined wild-type and mutant strains of Klebsiella aerogenes for the relative amounts of ribonucleic acid (RNA) hybridizing specifically to deoxyribonucleic acid from a transducing phage carrying glnAK, the structural gene for glutamine synthetase. Our data showed a positive correlation between the intracellular level of glutamine synthetase and the level of glnA messenger RNA; we were unable to detect glnA messinger RNA in strains devoid of glutamine synthetase protein. Therefore, it is possible that transcription of glnA is not regulated simply by repression mediated through the glutamine synthetase protein; rather, autogenous control in this system may involve activation of transcription. Our experiments also suggest that the promotor of the glnA gene is located at the rha proximal end of the gene.
我们检测了产气克雷伯菌的野生型和突变株，以确定与携带谷氨酰胺合成酶结构基因glnAK的转导噬菌体的脱氧核糖核酸特异性杂交的核糖核酸（RNA）的相对含量。我们的数据显示谷氨酰胺合成酶的细胞内水平与glnA信使RNA的水平呈正相关；在缺乏谷氨酰胺合成酶蛋白的菌株中，我们无法检测到glnA信使RNA。因此，glnA的转录可能不是简单地由谷氨酰胺合成酶蛋白介导的阻遏作用来调节；相反，该系统中的自体调控可能涉及转录激活。我们的实验还表明，glnA基因的启动子位于该基因的近端rha处。
