Tyler B M, Goldberg R B
J Bacteriol. 1976 Mar;125(3):1105-11. doi: 10.1128/jb.125.3.1105-1111.1976.
We have used P1 transduction to create intergeneric hybrid strains of enteric bacteria by moving the genA and hut genes between Klebsiella aerogenes, Escherichia coli and Salmonella typhimurium. The use of E. coli as the recipient in such transductions permits the construction of episomes and specialized transducing phage containing non-E. coli material. The effect of host restriction modification and deoxyribonucleic acid homology on the frequency of intergeneric transduction of these loci has been examined.
我们利用P1转导,通过在产气克雷伯菌、大肠杆菌和鼠伤寒沙门氏菌之间转移genA和hut基因,构建了肠道细菌的属间杂交菌株。在这种转导中使用大肠杆菌作为受体,能够构建含有非大肠杆菌物质的附加体和特异性转导噬菌体。我们还研究了宿主限制修饰和脱氧核糖核酸同源性对这些基因座属间转导频率的影响。