Brown W J, Sautter R L, Crist A E
Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan 48201.
Antimicrob Agents Chemother. 1992 Aug;36(8):1635-8. doi: 10.1128/AAC.36.8.1635.
Methylobacterium species represent a relatively new genus which is being increasingly isolated from cases of opportunistic infections. This study reports on 3 reference strains and 15 clinical isolates of Methylobacterium species. Susceptibility tests were performed by the agar dilution and commercial broth microdilution methods at both 30 and 35 degrees C. Readings were made at 24, 48, and 72 h. Incubation conditions of 48 h and 30 degrees C were found to be optimum. Both the agar dilution and broth microdilution methods gave equivalent results. Drugs tested and their MICs for 90% of isolates (in micrograms per milliliter) were as follows: amikacin, less than or equal to 1; gentamicin, 1; ciprofloxacin, 1; trimethoprim-sulfamethoxazole, 2/38; ceftriaxone, 16; and ceftizoxime, 16. The majority of our isolates were resistant to six other beta-lactam drugs tested. Nine of the 15 Methylobacterium isolates were beta-lactamase positive.
甲基杆菌属是一个相对较新的菌属，越来越多地从机会性感染病例中分离出来。本研究报告了3株甲基杆菌属参考菌株和15株临床分离株。采用琼脂稀释法和商业肉汤微量稀释法在30℃和35℃下进行药敏试验。在24、48和72小时读数。发现48小时和30℃的培养条件是最佳的。琼脂稀释法和肉汤微量稀释法得到的结果相当。所测试药物及其对90%分离株的最低抑菌浓度（以微克/毫升计）如下：阿米卡星，小于或等于1；庆大霉素，1；环丙沙星，1；甲氧苄啶-磺胺甲恶唑，2/38；头孢曲松，16；头孢唑肟，16。我们的大多数分离株对其他六种测试的β-内酰胺类药物耐药。15株甲基杆菌分离株中有9株β-内酰胺酶阳性。
