一种微技术在钩端螺旋体抗体凝集试验中的应用

APPLICATION OF A MICROTECHNIQUE TO THE AGGLUTINATION TEST FOR LEPTOSPIRAL ANTIBODIES.

作者信息

GALTON M M, SULZER C R, SANTAROSA C A, FIELDS M J

出版信息

Appl Microbiol. 1965 Jan;13(1):81-5. doi: 10.1128/am.13.1.81-85.1965.

DOI:10.1128/am.13.1.81-85.1965

PMID:14264852

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1058195/

Abstract

A microtechnique has been developed and adapted successfully to the microscopic agglutination test with live antigens for detection of leptospiral antibodies. Simultaneous titrations were performed by the conventional microscopic agglutination test and the microtechnique. When the microtechnique was used to screen 50 unknown leptospiral strains with a battery of hyperimmune sera, 98% agreement was obtained with the conventional procedure. Comparative data on 635 tests on these 50 cultures established the reliability of the microtechnique. Results with the two tests on 46 human sera revealed 93% agreement in the detection of leptospiral antibodies. The validity and reliability of the microtechnique obtained in these comparative studies suggests that it can be used as a valuable screening procedure for the microscopic agglutination test for preliminary cross agglutination studies on unknown strains and for the detection of leptospiral antibodies in human and animal sera.

摘要

已成功开发并改进了一种显微技术，用于以活抗原进行显微凝集试验，以检测钩端螺旋体抗体。通过传统显微凝集试验和显微技术进行同步滴定。当使用显微技术用一组超免疫血清筛选50株未知钩端螺旋体菌株时，与传统方法的一致性达98%。对这50种培养物进行的635次试验的比较数据证实了该显微技术的可靠性。对46份人血清进行的两种试验结果显示，在检测钩端螺旋体抗体方面的一致性为93%。这些比较研究中获得的显微技术的有效性和可靠性表明，它可作为一种有价值的筛选程序，用于显微凝集试验，以对未知菌株进行初步交叉凝集研究，并用于检测人和动物血清中的钩端螺旋体抗体。